Fourier transform tandem mass spectrometry (MS/MS) provides high mass accuracy, high sensitivity, and analytical versatility and has therefore emerged as an indispensable tool for structural elucidation of biomolecules. Glycosylation is one of the most common posttranslational modifications, occurring in ~50% of proteins. However, due to the structural diversity of carbohydrates, arising from non-template driven biosynthesis, achievement of detailed structural insight is highly challenging. This review briefly discusses carbohydrate sample preparation and ionization methods, and highlights recent developments in alternative high-resolution MS/MS strategies, including infrared multiphoton dissociation (IRMPD), electron capture dissociation (ECD), and electron detachment dissociation (EDD), for carbohydrates with a focus on glycans and proteoglycans from mammalian glycoproteins.

中文翻译：

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通过傅里叶变换串联质谱法表征碳水化合物的结构


傅里叶变换串联质谱 (MS/MS) 具有高质量精度、高灵敏度和分析多功能性，因此已成为生物分子结构阐明不可或缺的工具。糖基化是最常见的翻译后修饰之一，发生在约 50% 的蛋白质中。然而，由于碳水化合物的结构多样性是由非模板驱动的生物合成产生的，实现详细的结构洞察非常具有挑战性。本综述简要讨论了碳水化合物样品制备和电离方法，并重点介绍了碳水化合物的替代高分辨率 MS/MS 策略的最新发展，包括红外多光子解离 (IRMPD)、电子捕获解离 (ECD) 和电子脱离解离 (EDD)重点关注哺乳动物糖蛋白中的聚糖和蛋白聚糖。