Human seminal plasma contains a large array of proteins of clinical importance which are essentially needed to maintain the reproductive physiology of spermatozoa and for successful fertilization. Thus, isolation and identification of seminal plasma proteins is of paramount significance for their biophysical characterization and functional analysis in reproductive physiological processes. In this study, we have isolated Concanavalin-A binding glycoproteins from human seminal plasma and subsequently identified them by MALDI-TOF/MS analysis. The major proteins, as identified in this study, are Aminopeptidase N, lactoferrin, prostatic acid phosphatase, zinc-alpha-2-glycoprotein, prostate specific antigen, progestagen-associated endometrial protein, Izumo sperm-egg fusion protein and prolactin inducible protein. This paper also reports preliminary studies to identify altered expression of these proteins in oligospermia and azoospermia in comparison to normospermia. In oligospermia, five proteins were found to be downregulated while in azoospermia, four proteins were downregulated and two proteins were upregulated. Thus, this study is of immense biomedical interest towards identification of potential male infertility marker proteins in seminal plasma.

中文翻译：

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从人精浆中分离和鉴定伴刀豆球蛋白 A 结合糖蛋白：迈向鉴定男性不育标记蛋白的一步。

人类精浆含有大量具有临床重要性的蛋白质，它们是维持精子生殖生理和成功受精所必需的。因此，精浆蛋白的分离和鉴定对于它们在生殖生理过程中的生物物理表征和功能分析具有极其重要的意义。在这项研究中，我们从人精浆中分离了伴刀豆球蛋白 A 结合糖蛋白，随后通过 MALDI-TOF/MS 分析对其进行了鉴定。本研究中确定的主要蛋白质是氨肽酶 N、乳铁蛋白、前列腺酸性磷酸酶、锌-α-2-糖蛋白、前列腺特异性抗原、孕激素相关子宫内膜蛋白、出云精卵融合蛋白和催乳素诱导蛋白。本文还报告了初步研究，以确定与正常精子症相比，这些蛋白质在少精子症和无精子症中的表达改变。在少精子症中，发现五种蛋白质被下调，而在无精子症中，四种蛋白质被下调，两种蛋白质被上调。因此，这项研究对于鉴定精浆中潜在的男性不育标记蛋白具有巨大的生物医学意义。