MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

中文翻译：

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表达 MuSI 基因的烟草植物的镉抗性。

MuSI是一种与包含橡胶伸长因子 (REF) 的结构域相对应的基因，与植物中的许多胁迫相关蛋白高度同源。由于MuSI在用镉或铜处理的植物根中上调，因此本研究调查了MuSI与镉耐受性的关系。与单独用载体转染的野生型细胞相比，过表达MuSI 的大肠杆菌细胞对 Cd 的抵抗力更强。MuSI转基因植物也更能抵抗 Cd。信息系统转基因烟草植物比野生型植物吸收更少的 Cd。在转基因植物中，从根到芽的 Cd 易位减少，从而避免了 Cd 毒性。镉处理增加了野生型烟草植物叶片中短毛状体的数量，而在MuSI转基因烟草中没有变化。这些结果表明，MuSI转基因烟草植物通过减少 Cd 吸收和/或增加根中 Cd 的固定来增强对 Cd 的耐受性，导致较少的 Cd 易位到枝条。