Lecitase® Ultra, a phospholipase, was explored as an effective biocatalyst for direct esterification of glycerol with oleic acid to produce 1,3-DAG. Experiments were carried out in batch mode, and optimal reaction conditions were evaluated. In comparison with several organic solvent mediums, the solvent-free system was found to be more beneficial for this esterification reaction, which was further studied to investigate the reaction conditions including oleic acid/glycerol mole ratio, temperature, initial water content, enzyme load, and operating time. The results showed that Lecitase® Ultra catalyzed a fast synthesis of 1,3-DAG by direct esterification in a solvent-free medium. Under the optimal reaction conditions, a short reaction time 1.5 h was found to achieve the fatty acid esterification efficiency of 80.3 ± 1.2% and 1,3-DAG content of 54.8 ± 1.6 wt% (lipid layer of reaction mixture mass). The reusability of Lecitase® Ultra was evaluated via recycling the excess glycerol layer in the reaction system. DAG in the upper lipid layer of reaction mixture was purified by molecular distillation and the 1,3-DAG-enriched oil with a purity of about 75 wt% was obtained. Practical applications: The new Lecitase® Ultra catalyzed process for production of 1,3-DAG from glycerol and oleic acid described in this study provides several advantages over conventional methods including short reaction time, the absence of a solvents and a high product yield.

中文翻译：

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在无溶剂体系中通过 Lecitase® 超催化酯化快速合成 1,3-DAG

Lecitase® Ultra 是一种磷脂酶，被研究作为一种有效的生物催化剂，用于甘油与油酸的直接酯化以产生 1,3-DAG。实验以分批模式进行，并评估最佳反应条件。与几种有机溶剂介质相比，发现无溶剂体系更有利于这种酯化反应，进一步研究了反应条件，包括油酸/甘油摩尔比、温度、初始含水量、酶负荷、和操作时间。结果表明，Lecitase® Ultra 在无溶剂介质中通过直接酯化作用催化 1,3-DAG 的快速合成。在最佳反应条件下，发现较短的反应时间 1.5 h 即可实现 80.3 ± 1.2% 的脂肪酸酯化效率和 54 的 1,3-DAG 含量。8±1.6wt%（反应混合物质量的脂质层）。Lecitase® Ultra 的可重复使用性是通过回收反应系统中多余的甘油层来评估的。通过分子蒸馏纯化反应混合物上脂质层中的DAG，得到纯度约75wt％的富含1,3-DAG的油。实际应用：本研究中描述的用于从甘油和油酸生产 1,3-DAG 的新型 Lecitase® Ultra 催化工艺与传统方法相比具有多项优势，包括反应时间短、不使用溶剂和产品收率高。通过分子蒸馏纯化反应混合物上脂质层中的DAG，得到纯度约75wt％的富含1,3-DAG的油。实际应用：本研究中描述的用于从甘油和油酸生产 1,3-DAG 的新型 Lecitase® Ultra 催化工艺与传统方法相比具有多种优势，包括反应时间短、无溶剂和产品收率高。通过分子蒸馏纯化反应混合物上脂质层中的DAG，得到纯度约75wt％的富含1,3-DAG的油。实际应用：本研究中描述的用于从甘油和油酸生产 1,3-DAG 的新型 Lecitase® Ultra 催化工艺与传统方法相比具有多种优势，包括反应时间短、不使用溶剂和产品收率高。