In this study, we have mapped the surface charge of wool fibers using chemically specific high-resolution force spectroscopy in order to better understand the dispersion of amino acids in relation to fiber morphology. The inter-surface forces between standard atomic force microscopy (AFM) probe tips (tip radius ~ 50 nm) functionalized with COOH and NH(3) terminated alkanethiol self assembling monolayers and the wool surface were used to estimate the surface charge per unit area using linear Poisson-Boltzmann-based electrostatic double layer theory. The positional measurement of nano-scale surface charge showed a correlation between the surface charge and fiber morphology, indicated that basic amino acids are located near the scale edges.

中文翻译：

---

通过高分辨率力谱评估沿羊毛纤维表面的表面电荷变化。

在这项研究中，我们使用化学特异性高分辨率力谱绘制了羊毛纤维的表面电荷，以更好地了解氨基酸的分散与纤维形态的关系。标准原子力显微镜 (AFM) 探针尖端（尖端半径 ~ 50 nm）与 COOH 和 NH(3) 终止的链烷硫醇自组装单分子层和羊毛表面之间的表面间力用于估计每单位面积的表面电荷使用基于线性 Poisson-Boltzmann 的静电双层理论。纳米级表面电荷的位置测量表明表面电荷与纤维形态之间存在相关性，表明碱性氨基酸位于鳞片边缘附近。