Some studies have demonstrated the involvement of nuclear factor-kappa B (NF-kB) in the pathogenesis of glomerulonephritis. The aim of our study was twofold: (1) to analyze the prognostic value of NF-kB expression in primary IgA nephropathy (IgAN) and (2) to compare the results of NF-kB expression by immunohistochemistry (IHC) and southwestern histochemistry (SWH). We analyzed 62 patients diagnosed with IgAN from 1987 to 2003. We used monoclonal antibodies to CD68 and mast cell tryptase and polyclonal antibodies to TGF-β1, α-SMA and NF-kB p65. We used SWH for the in situ detection of activated NF-kB. The results showed that NF-kB expression (mainly by SWH) correlated with clinical and histological parameters. An unfavorable clinical course of IgAN was significantly related to tubular NF-kB expression by SWH, but not by IHC. The Kaplan-Meier curves demonstrated that increased NF-kB expression, which was measured by IHC and SWH, decreased renal survival. In conclusion, the increased expression of NF-kB in the tubular area may be a predictive factor for the poor prognosis of patients with IgAN. Compared with IHC, NF-kB expression determined by SWH was correlated with a larger number of parameters of poor disease outcome.

中文翻译：

---

IgA 肾病结果中的 NF-kB 表达。

一些研究表明核因子-κB (NF-kB) 参与肾小球肾炎的发病机制。我们研究的目的有两个：（1）分析 NF-kB 表达在原发性 IgA 肾病（IgAN）中的预后价值；（2）比较免疫组织化学（IHC）和西南组织化学的 NF-kB 表达结果。 SWH）。我们分析了 1987 年至 2003 年间诊断为 IgAN 的 62 名患者。我们使用了 CD68 和肥大细胞类胰蛋白酶的单克隆抗体以及 TGF-β1、α-SMA 和 NF-kB p65 的多克隆抗体。我们使用 SWH 进行原位检测激活的 NF-kB。结果表明，NF-kB 表达（主要通过 SWH）与临床和组织学参数相关。IgAN 的不利临床过程与 SWH 的肾小管 NF-kB 表达显着相关，但与 IHC 无关。Kaplan-Meier 曲线表明，通过 IHC 和 SWH 测量的 NF-kB 表达增加会降低肾脏存活率。综上所述，肾小管区NF-kB表达增加可能是IgAN患者预后不良的预测因素。与 IHC 相比，由 SWH 确定的 NF-kB 表达与大量不良疾病结果的参数相关。