The lipids of alga Scenedesmus obliquus grown under controlled conditions were separated and fractionated by column and thin-layer chromatography, and fatty acid composition of each lipid component was studied by gas-liquid chromatography (GLC). Total lipids were 11.17%, and neutral lipid, glycolipid and phospholipid fractions were 7.24%, 2.45% and 1.48% on a dry weight basis, respectively. The major neutral lipids were diglycerides, triglycerides, free sterols, hydrocarbons and sterol esters. The glycolipids were: monogalactosyl diglyceride, digalactosyl diglyceride, esterified sterol glycoside, and sterol glycoside. The phospholipids included: phosphatidyl choline, phosphatidyl glycerol and phosphatidyl ethanolamine. Fourteen fatty acids were identified in the four lipid fractions by GLC. The main fatty acids were C18:2, C16:0, C18:3(alpha), C18:1, C16:3, C16:1, and C16:4. Total unsaturated fatty acid and essential fatty acid compositions of the total algal lipids were 80% and 38%, respectively.

中文翻译：

---

在恒定细胞密度装置中生长的绿藻的脂质含量和脂肪酸组成

通过柱层析和薄层层析将在受控条件下生长的藻类Scenedesmus obliquus 的脂质进行分离和分级，并通过气液色谱(GLC) 研究每种脂质成分的脂肪酸组成。按干重计，总脂质为 11.17%，中性脂质、糖脂和磷脂组分分别为 7.24%、2.45% 和 1.48%。主要的中性脂质是甘油二酯、甘油三酯、游离甾醇、碳氢化合物和甾醇酯。糖脂是：单半乳糖甘油二酯、双半乳糖甘油二酯、酯化甾醇糖苷和甾醇糖苷。磷脂包括：磷脂酰胆碱、磷脂酰甘油和磷脂酰乙醇胺。通过 GLC 在四种脂质组分中鉴定出 14 种脂肪酸。主要脂肪酸为 C18:2、C16:0、C18:3(alpha)、C18: 1、C16:3、C16:1 和 C16:4。总不饱和脂肪酸和必需脂肪酸组成的总藻类脂质分别为80%和38%。