The karyotype of Greek cobitid fish Cobitisstrumicae Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA3 fluorescence banding and also by in situ hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA3 staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Bicanestrinia Băcescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Cobitis Linnaeus, 1758 are further discussed.

中文翻译：

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希腊沃尔维湖 Cobitisstrumicae Karaman（Teleostei，Cobitidae）rDNA 的核型和染色体特征，1955。

通过连续吉姆萨染色、C 显带、银染、CMA3 荧光显带对来自希腊沃尔维湖的希腊鳅鱼 Cobitisstrumicae Karaman（1955 年）的核型进行了分析，它是其两个主要种内系统发育谱系之一的代表。也可通过 rDNA 探针进行原位杂交 (FISH)。二倍体染色体数目为2n = 50，核型由10对中着丝粒至亚中着丝粒染色体和15对亚端着丝粒至近端着丝粒染色体组成。通过 Ag 和 CMA3 染色以及 FISH 显示的核仁组织区 (NOR) 位于第四个亚中着丝粒染色体对的端粒区域。染色体中 C 阳性异染色质含量非常低。未检测到异形性染色体。1962 年，这是 Bicanestrinia Băcescu 谱系任何物种的第一份核型报告，显示了以近端着丝粒染色体为主的简单核型，并拥有单个 NOR 轴承染色体对。进一步讨论了这一发现对 Cobitis Linnaeus, 1758 属分类学的细胞分类学意义。