In this study, a nanobiosensor for detecting explosives was developed, in which the peptide was synthesized with trinitrotoluene (TNT)-specific sequence and immobilized on nanodevice by Au-S covalent linkage, and the nanocup arrays were fabricated by nanoimprint and deposited with Au nanoparticles to generate localized surface plasmon resonance (LSPR). The device was used to monitor slight change from specific binding of 2,4,6-TNT to the peptide. With high refractive index sensing of ~104 nm/RIU, the nanocup device can detect the binding of TNT at concentration as low as 3.12 × 10-7 mg mL-1 by optical transmission spectrum modulated by LSPR. The nanosensor is also able to distinguish TNT from analogs of 2,4-dinitrotoluene and 3-nitrotoluene in the mixture with great selectivity. The peptide-based nanosensor provides novel approaches to design versatile biosensor assays by LSPR for chemical molecules.

中文翻译：

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用于爆炸物检测的肽功能化纳米等离子体传感器。

本研究开发了一种用于检测爆炸物的纳米生物传感器，其中肽以三硝基甲苯（TNT）特异性序列合成，并通过Au-S共价键固定在纳米器件上，通过纳米压印技术制造纳米杯阵列并沉积有Au纳米颗粒产生局域表面等离子体共振（LSPR）。该装置用于监测 2,4,6-TNT 与肽的特异性结合的轻微变化。纳米杯装置具有约 104 nm/RIU 的高折射率传感，可以通过 LSPR 调制的光学透射光谱检测浓度低至 3.12 × 10-7 mg mL-1 的 TNT 结合。该纳米传感器还能够以极高的选择性将 TNT 与混合物中的 2,4-二硝基甲苯和 3-硝基甲苯类似物区分开来。基于肽的纳米传感器提供了通过局域表面等离子体共振（LSPR）针对化学分子设计多功能生物传感器测定的新方法。