C60 fullerene-based nanoformulations are proposed to have a direct toxic effect on tumor cells. Previous investigations demonstrated that C60 fullerene used alone or being conjugated with chemotherapeutic agents possesses a potent anticancer activity. The main aim of this study was to investigate the effect of C60 fullerene and its nanocomplexes with anticancer drugs on human phagocyte metabolic profile in vitro. Analysis of the metabolic profile of phagocytes exposed to C60 fullerene in vitro revealed augmented phagocytic activity and down-regulated reactive nitrogen species generation in these cells. Additionally, cytofluorimetric analysis showed that C60 fullerene can exert direct cytotoxic effect on normal and transformed phagocytes through the vigorous induction of intracellular reactive oxygen species generation. Cytotoxic action as well as the pro-oxidant effect of C60 fullerene was more pronounced toward malignant phagocytes. At the same time, C60 fullerenes have the ability to down-regulate the pro-oxidant effect of cisplatin on normal cells. These results indicate that C60 fullerenes may influence phagocyte metabolism and have both pro-oxidant and antioxidant properties. The antineoplastic effect of C60 fullerene has been observed by direct toxic effect on tumor cells, as well as through the modulation of the functions of effector cells of antitumor immunity.

中文翻译：

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C60 富勒烯及其与抗癌药物的纳米复合物可调节循环吞噬细胞功能并显着增加转化单核细胞中 ROS 的产生。

提出基于 C60 富勒烯的纳米制剂对肿瘤细胞具有直接毒性作用。先前的研究表明，单独使用或与化学治疗剂结合使用的 C60 富勒烯具有有效的抗癌活性。本研究的主要目的是研究 C60 富勒烯及其纳米复合物与抗癌药物对体外人体吞噬细胞代谢谱的影响。体外暴露于 C60 富勒烯的吞噬细胞的代谢特征分析显示，这些细胞的吞噬活性增强，活性氮种类的产生下调。此外，细胞荧光分析表明，C60 富勒烯可以通过强烈诱导细胞内活性氧的产生，对正常和转化的吞噬细胞产生直接的细胞毒作用。C60富勒烯的细胞毒作用和促氧化作用对恶性吞噬细胞更为明显。同时，C60富勒烯具有下调顺铂对正常细胞的促氧化作用的能力。这些结果表明，C60 富勒烯可能影响吞噬细胞代谢并具有促氧化和抗氧化特性。已通过对肿瘤细胞的直接毒性作用以及通过调节抗肿瘤免疫效应细胞的功能观察到C60富勒烯的抗肿瘤作用。这些结果表明，C60 富勒烯可能影响吞噬细胞代谢并具有促氧化和抗氧化特性。已通过对肿瘤细胞的直接毒性作用以及通过调节抗肿瘤免疫效应细胞的功能观察到C60富勒烯的抗肿瘤作用。这些结果表明，C60 富勒烯可能影响吞噬细胞代谢并具有促氧化和抗氧化特性。已通过对肿瘤细胞的直接毒性作用以及通过调节抗肿瘤免疫效应细胞的功能观察到C60富勒烯的抗肿瘤作用。