Aim: The knowledge of cellular proteins that involves cell cycle and its control system is essential for understanding tumor biology. Minichromosome maintenance protein (Mcm-2), a component of prereplicative complex, essential for initiating DNA replication, is deregulated in different malignant lesions, and is expressed throughout the whole cell cycle including the G0 and G1 phases. This characteristic cell cycle event is not found in other proliferative markers such as geminin, AgNOR, Ki-67, and proliferating cell nuclear antigen. The aim of the present study was to analyze and compare the expression of Mcm-2 in normal oral mucosa (NM) and oral squamous cell carcinomas at tumor margins (TM), the tumor center (TC), and the invasive tumor front (ITF), with correlation of clinicopathologic features. Materials and Methods: Tissues from 50 oral squamous cell carcinomas and 10 NM were archived retrospectively and stained with an antibody directed against the Mcm-2 antigen. A quantitative method was used to score the Mcm-2 expression in NM, TM, TC, and ITF. Nuclei labeling index for each case was estimated as the percentage of immunoreactive nuclei among 500 cells separately for NM, TM, TC, and ITF. Results: Nuclei labeling index increases progressively from NM (49.08%), TM (67.79%), and TC (76.87%) to ITF (87.77%). Conclusions: Cell proliferation by Mcm-2 at the ITF had a strong positive relationship with TC, TM. Mcm-2, a pan-cell cycle marker, is more sensitive in comparison with other conventional proliferative markers, which can be a better prognostic indicator.

中文翻译：

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Mcm-2辅助评估口腔鳞状细胞癌的肿瘤边缘、肿瘤中心和浸润性肿瘤前沿之间的增殖指数

目的：涉及细胞周期及其控制系统的细胞蛋白质的知识对于理解肿瘤生物学至关重要。微染色体维持蛋白 (Mcm-2) 是复制前复合物的一种成分，对启动 DNA 复制至关重要，在不同的恶性病变中被解除调节，并在包括 G0 和 G1 期在内的整个细胞周期中表达。这种特征性的细胞周期事件在其他增殖标记物中没有发现，例如 geminin、AgNOR、Ki-67 和增殖细胞核抗原。本研究的目的是分析和比较 Mcm-2 在正常口腔黏膜 (NM) 和口腔鳞状细胞癌中肿瘤边缘 (TM)、肿瘤中心 (TC) 和浸润性肿瘤前沿 (ITF) 的表达。 )，与临床病理特征的相关性。材料和方法：对来自 50 个口腔鳞状细胞癌和 10 个 NM 的组织进行回顾性存档，并用针对 Mcm-2 抗原的抗体染色。使用定量方法对 NM、TM、TC 和 ITF 中的 Mcm-2 表达进行评分。每个病例的细胞核标记指数估计为 NM、TM、TC 和 ITF 的 500 个细胞中免疫反应性细胞核的百分比。结果：核标记指数从 NM (49.08%)、TM (67.79%) 和 TC (76.87%) 到 ITF (87.77%) 逐渐增加。结论： Mcm-2 在 ITF 的细胞增殖与 TC、TM 有很强的正相关关系。Mcm-2 是一种泛细胞周期标志物，与其他常规增殖标志物相比更敏感，可以成为更好的预后指标。