Cardiovascular diseases cause huge socio-economic burden worldwide. Although a mammalian myocardium has its own limited healing capability, scaffold materials capable of releasing stem cell recruiting/engrafting factors may facilitate the regeneration of the infarcted myocardium. The aim of this research was to develop cardiac patches capable of simultaneously eluting substance P (SP) and insulin-like growth factor-1C (IGF-1C) peptide. Polycaprolactone/collagen type 1-based patches with or without SP and IGF-1C peptide were fabricated by co-electrospinning, which exhibited nanofibrous morphology. SP and IGF-1C/SP patches recruited significantly higher numbers of bone marrow-mesenchymal stem cells than that of the negative control and patch-only groups in vitro. The developed patches were transplanted in an infarcted myocardium for up to 14 days. Mice underwent left anterior descending artery ligation and received one of the following treatments: (i) sham, (ii) saline, (iii) patch-only, (iv) IGF-1C patch, (v) SP patch and (vi) IGF-1C/SP patch. SP and IGF-1C/SP patch-treated groups exhibited better heart function and attenuated adverse cardiac remodeling than that of the saline, patch-only and individual peptide containing cardiac patches. SP patch and IGF-1C/SP patch-treated groups also showed higher numbers of CD31-positive vessels and isolectin B4-positive capillaries than that of other groups. IGF-1C/SP-treated group also showed thicker left ventricular wall in comparison to the saline and patch-only groups. Moreover, IGF-1C/SP patches recruited significantly higher numbers of CD29-positive cells and showed less numbers of Tunel-positive cells compared with the other groups. These data suggest that SP and IGF-1C peptides may act synergistically for in situ tissue repair.

中文翻译：

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使用神经肽 P 物质和 IGF-1C 肽洗脱心脏贴片进行原位心脏再生。

心血管疾病在全世界造成巨大的社会经济负担。尽管哺乳动物心肌自身的愈合能力有限，但能够释放干细胞募集/移植因子的支架材料可以促进梗塞心肌的再生。本研究的目的是开发能够同时洗脱 P 物质 (SP) 和胰岛素样生长因子-1C (IGF-1C) 肽的心脏贴片。通过共静电纺丝制备了含有或不含 SP 和 IGF-1C 肽的基于聚己内酯/1 型胶原蛋白的贴片，其表现出纳米纤维形态。在体外，SP 和 IGF-1C/SP 贴片募集的骨髓间充质干细胞数量显着高于阴性对照组和仅贴片组。将开发的贴片移植到梗塞心肌中长达 14 天。小鼠接受左前降支结扎并接受以下治疗之一：（i）假手术，（ii）盐水，（iii）仅贴片，（iv）IGF-1C贴片，（v）SP贴片和（vi）IGF -1C/SP 补丁。与盐水、仅贴片和含有单独肽的心脏贴片相比，SP和IGF-1C/SP贴片治疗组表现出更好的心脏功能并减轻了不良心脏重塑。SP贴片和IGF-1C/SP贴片治疗组也显示出比其他组更多的CD31阳性血管和异凝集素B4阳性毛细血管数量。与盐水组和仅贴片组相比，IGF-1C/SP 治疗组还显示出更厚的左心室壁。此外，与其他组相比，IGF-1C/SP 贴片招募的 CD29 阳性细胞数量明显增多，而 Tunel 阳性细胞数量较少。这些数据表明 SP 和 IGF-1C 肽可能在原位组织修复中发挥协同作用。