ABSTRACT Bioaugmentation in the acid-phase of a two-phase anaerobic membrane bioreactor (AnMBR) treating primary sludge was investigated as a means for targeting and improving hydrolysis and acetogenesis. Bioaugmentation was carried out using a proprietary bioculture blend containing a mixture of hydrolytic, acidogenic, and acetogenic microorganisms. This mixture was added on its own and in combination with recycled anaerobic sludge from the methane-phase of the AnMBR. Both bioaugmentation strategies had a positive effect on overall hydrolysis (25–38%), and acid-phase acetic acid generation (31–52%) compared to operation without bioaugmentation. This led to subsequent increases in average methane production (10–13%), and greater average solids reduction (25–55%). Microbial community analysis using 16S Illumina MiSeq generated sequences revealed increased relative abundance of Acetobacter and Syntrophomonas species in bioaugmented communities, suggesting these to be key players in improvements in process performance. However, in general the relative abundance of bioaugmented microorganisms within bioaugmented communities was relatively low, highlighting the need to optimize the bioculture composition and dosage. Overall, bioaugmentation was found to benefit the conversion of primary sludge to methane, when initial solubility was relatively low. Future work should optimize the bioculture composition and dosing strategy to improve its effectiveness and long-term stability, and minimize associated operating costs. GRAPHICAL ABSTRACT

中文翻译：

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利用生物强化提高两相 AnMBR 处理污水污泥的性能

摘要 研究了处理初级污泥的两相厌氧膜生物反应器 (AnMBR) 的酸相中的生物强化作为靶向和改善水解和产乙酸的手段。使用含有水解、产酸和产乙酸微生物混合物的专有生物培养混合物进行生物强化。该混合物单独添加，并与来自 AnMBR 甲烷相的循环厌氧污泥结合添加。与没有生物强化的操作相比，两种生物强化策略对整体水解（25-38%）和酸相乙酸生成（31-52%）都有积极影响。这导致随后平均甲烷产量增加（10-13%），以及更大的平均固体减少量（25-55%）。使用 16S Illumina MiSeq 生成的序列进行的微生物群落分析显示，生物增强群落中醋杆菌和合成单胞菌物种的相对丰度增加，表明它们是改善工艺性能的关键因素。然而，一般而言，生物增强群落内生物增强微生物的相对丰度相对较低，突出了优化生物培养组成和剂量的必要性。总体而言，当初始溶解度相对较低时，发现生物强化有利于初级污泥转化为甲烷。未来的工作应该优化生物培养的组成和剂量策略，以提高其有效性和长期稳定性，并最大限度地减少相关的运营成本。图形概要 总的来说，生物强化群落内生物强化微生物的相对丰度相对较低，突出了优化生物培养组成和剂量的必要性。总体而言，当初始溶解度相对较低时，发现生物强化有利于初级污泥转化为甲烷。未来的工作应该优化生物培养组成和剂量策略，以提高其有效性和长期稳定性，并最大限度地减少相关的运营成本。图形概要 总的来说，生物强化群落内生物强化微生物的相对丰度相对较低，突出了优化生物培养组成和剂量的必要性。总体而言，当初始溶解度相对较低时，发现生物强化有利于初级污泥转化为甲烷。未来的工作应该优化生物培养组成和剂量策略，以提高其有效性和长期稳定性，并最大限度地减少相关的运营成本。图形概要 未来的工作应该优化生物培养组成和剂量策略，以提高其有效性和长期稳定性，并最大限度地减少相关的运营成本。图形概要 未来的工作应该优化生物培养组成和剂量策略，以提高其有效性和长期稳定性，并最大限度地减少相关的运营成本。图形概要