Meso- and macroporous spherical silica gels of pore sizes in the range of 60–1000 Å and 40–75 µm particle size were investigated as a stationary phase for the separation and purification of polysaccharides and poly(ethylene glycols) (PEGs) of various MWs using an aqueous mobile phase. Sephadex and Bio-Gel were used for comparison as the most common stationary phases for similar purposes. The separation of dextrans of a mean MW = 31 kDa from small molecules (NaCl) was possible with SiO2 with a pore size of 60–300 Å, but the observed efficiencies of a column of the same size were lower comparing with Sephadex or Bio-Gel. In the case of oxidized alginic acid only SiO2 of the 60 Å pore size was suitable, while Sephadex, Bio-Gel and other investigated silicas were not efficient. Sephadex and 300–1000 Å SiO2 offered the possibility of dividing dextrans with MW within the range of 1 MDa–10 kDa into fractions of various MWs, while Bio-Gel and 60 Å SiO2 were not suitable. The investigated silica gels strongly adsorbed PEGs of MW 2–20 kDa. The amount adsorbed decreased with the increase of pore size and they were not useful as a stationary phase for this class of polymers. An advantage of SiO2 of the investigated particle size was a very low back pressure comparing with Sephadex. A considerably lower price of silica offers time- and cost-efficient separation of polysaccharides.Graphical Abstract

中文翻译：

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使用大孔球形硅胶作为固定相进行多糖的 SEC 分离

研究了孔径在 60-1000 Å 和 40-75 µm 范围内的中孔和大孔球形硅胶作为分离和纯化各种分子量的多糖和聚乙二醇 (PEG) 的固定相使用水性流动相。Sephadex 和 Bio-Gel 被用作用于类似目的的最常见固定相进行比较。使用孔径为 60–300 Å 的 SiO2 可以从小分子 (NaCl) 中分离平均 MW = 31 kDa 的葡聚糖，但与 Sephadex 或 Bio-凝胶。在氧化海藻酸的情况下，只有孔径为 60 Å 的 SiO2 是合适的，而 Sephadex、Bio-Gel 和其他研究的二氧化硅效率不高。Sephadex 和 300–1000 Å SiO2 提供了将 MW 在 1 MDa–10 kDa 范围内的葡聚糖分成不同 MW 级分的可能性，而 Bio-Gel 和 60 Å SiO2 则不适合。所研究的硅胶强烈吸附 MW 2-20 kDa 的 PEG。吸附量随着孔径的增加而减少，它们不能用作这类聚合物的固定相。与 Sephadex 相比，SiO2 的研究粒度的一个优点是背压非常低。二氧化硅的相当低的价格提供了时间和成本有效的多糖分离。图形摘要 吸附量随着孔径的增加而减少，它们不能用作这类聚合物的固定相。与 Sephadex 相比，SiO2 的研究粒度的一个优点是背压非常低。二氧化硅的相当低的价格提供了时间和成本有效的多糖分离。图形摘要 吸附量随着孔径的增加而减少，它们不能用作这类聚合物的固定相。与 Sephadex 相比，SiO2 的研究粒度的一个优点是背压非常低。二氧化硅的相当低的价格提供了时间和成本有效的多糖分离。图形摘要