ABSTRACT A major constraint in the microalgal technology is the economics involved in cultivation and harvesting. This work is focussed on the optimization of nutrients for cultivation and harvesting using ‘Scenedesmus sp’. Response surface methodology (RSM) using ‘Face centered central composite design’ (FCCD) available in Design expert 10.0.4 was used to develop the regression model for optimization of nutrients and flocculation conditions. The optimum nutrient conditions were 500 ppm of urea, 250 ppm of potassium dihydrogen phosphate and 1000 ppm of potassium hydrogen carbonate under artificial light conditions and 500 ppm of urea and 2000 ppm of potassium hydrogen carbonate under sunlight conditions. The optimum conditions were predicted using the model and compared with experimental data. The model has an R2 value of 0.9769 and 0.9798 for artificial light and sunlight conditions, respectively. In the case of harvesting studies, 98% flocculation efficiency was obtained for a combination of pH 10.4, temperature 45°C, 200 mg/l of leaf powder of Cassia auriculata. The model has an R2 value of 0.9989. The present studies indicated that cultivation of Scenedesmus sp. with the optimized nutrients and harvesting conditions facilitate a platform for energy efficient mass cultivation. GRAPHICAL ABSTRACT

中文翻译：

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栅藻的培养。使用优化的最少养分和絮凝剂——大规模培养的潜在平台。

摘要 微藻技术的一个主要制约因素是涉及种植和收获的经济性。这项工作的重点是使用 'Scenedesmus sp' 优化栽培和收获的养分。使用设计专家 10.0.4 中可用的“以面为中心的中心复合设计”(FCCD) 的响应面方法 (RSM) 用于开发回归模型以优化营养物和絮凝条件。最佳养分条件是人工光照条件下尿素500 ppm、磷酸二氢钾250 ppm和碳酸氢钾1000 ppm，日光条件下尿素500 ppm、碳酸氢钾2000 ppm。使用该模型预测最佳条件并与实验数据进行比较。该模型的 R2 值为 0.9769 和 0。9798 分别用于人造光和阳光条件。在收获研究的情况下，在 pH 10.4、温度 45°C、200 mg/l 决明子叶粉的组合下获得 98% 的絮凝效率。该模型的 R2 值为 0.9989。目前的研究表明，栅藻的培养。优化的养分和收获条件促进了高效节能的大规模种植平台。图形概要 优化的养分和收获条件促进了高效节能的大规模种植平台。图形概要 优化的养分和收获条件促进了高效节能的大规模种植平台。图形概要