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First cytogenetic analysis of lesser gymnures (Mammalia, Galericidae, Hylomys) from Vietnam.
Comparative Cytogenetics ( IF 1.0 ) Pub Date : 2018-10-03 , DOI: 10.3897/compcytogen.v12i3.27207
Svetlana V Pavlova 1 , Larisa S Biltueva 2 , Svetlana A Romanenko 2, 3 , Natalya A Lemskaya 2 , Anton V Shchinov 1 , Alexei V Abramov 4 , Viatcheslav V Rozhnov 1, 5
Affiliation  

Gymnures are an ancient group of small insectivorous mammals and are characterized by a controversial taxonomic status and the lack of a description of karyotypes for certain species. In this study, conventional cytogenetic techniques (Giemsa, CBG- and GTG-banding, Ag-NOR), CMA3-DAPI staining, and fluorescent in situ hybridization (FISH) with telomeric DNA probes were used to examine for the first time the karyotypes of lesser gymnures of group Hylomyssuillus Müller, 1840 from northern and southern Vietnam. All studied specimens had karyotypes with 2n=48, NFa=64. C-positive heterochromatic blocks existed in centromeric regions of 7 bi-armed autosomes and the submetacentric X chromosome. The Y chromosome is a C-positive and dot-like. The nucleolus organizer regions resided terminally on the short arms of 2 small bi-armed pairs. Positive signals at the telomeres of all chromosomes were revealed by FISH. CMA3-positive blocks were localized on the telomeric and pericentric regions of most bi-armed and acrocentric chromosomes. Despite the large genetic distances between Hylomys Müller, 1840, lesser gymnures from H.suillus-group from northern and southern Vietnam have similar karyotypic characteristics.

中文翻译:

越南小体操运动员(哺乳动物,galericidae,Hylomys)的首次细胞遗传学分析。

肉食动物是一个古老的小型食虫哺乳动物群体,其特征是有争议的生物分类地位,并且缺乏某些物种的核型描述。在这项研究中,传统的细胞遗传学技术(吉姆萨,CBG和GTG带,Ag-NOR),CMA3-DAPI染色和端粒DNA探针荧光原位杂交(FISH)被用来首次检查核型的核型。 HylomyssuillusMüller组的小型体操运动员,1840年,来自越南北部和南部。所有研究标本的核型均为2n = 48,NFa = 64。C阳性异色块存在于7个双臂常染色体的着丝粒区域和亚超中心X染色体中。Y染色体是C阳性且呈点状。核仁组织者区域末端位于2个小双臂对的短臂上。FISH显示所有染色体的端粒上的阳性信号。CMA3阳性块位于大多数双臂和acrocentric染色体的端粒和周围区域。尽管HylomysMüller(1840年)之间存在很大的遗传距离,但来自越南北部和南部的H.suillus-group较少的体操运动员具有相似的核型特征。
更新日期:2019-11-01
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