Pul-down assay is a popular in vitro method for identification of physical interactors of selected proteins. Here, for the first time, we compared three conventional variants of pull-down assay with the streptavidin-modified surface plasmon resonance (SPR) chips for the detection of PDZ and LIM domain protein 2 (PDLIM2) interaction partners.

中文翻译：

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基于SWATH-MS的Interomomics的链霉亲和素磁珠和表面等离振子共振芯片的下拉分析。

下拉测定法是一种流行的体外方法，用于鉴定所选蛋白质的物理相互作用物。在这里，我们第一次比较了下拉测定法的三种常规变体与链霉亲和素修饰的表面等离振子共振（SPR）芯片，用于检测PDZ和LIM域蛋白2（PDLIM2）的相互作用伙伴。