One of the most important cucumber diseases is bacterial angular leaf spot (ALS), whose increased occurrence in open-field production has been observed over the last years. To map ALS resistance genes, a recombinant inbred line (RIL) mapping population was developed from a narrow cross of cucumber line Gy14 carrying psl resistance gene and susceptible B10 line. Parental lines and RILs were tested under growth chamber conditions as well as in the field for angular leaf spot symptoms. Based on simple sequence repeat and DArTseq, genotyping a genetic map was constructed, which contained 717 loci in seven linkage groups, spanning 599.7 cM with 0.84 cM on average between markers. Monogenic inheritance of the lack of chlorotic halo around the lesions, which is typical for ALS resistance and related with the presence of recessive psl resistance gene, was confirmed. The psl locus was mapped on cucumber chromosome 5. Two major quantitative trait loci (QTL) psl5.1 and psl5.2 related to disease severity were found and located next to each other on chromosome 5; moreover, psl5.1 was co-located with psl locus. Identified QTL were validated in the field experiment. Constructed genetic map and markers linked to ALS resistance loci are novel resources that can contribute to cucumber breeding programs.

中文翻译：

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黄瓜 (Cucumis sativus L.) 抗角斑病的 psl 基因座和数量性状基因座的遗传图谱。

最重要的黄瓜病害之一是细菌性角斑病（ALS），近年来在露地生产中观察到该病的发生率有所增加。为了绘制 ALS 抗性基因图谱，我们利用携带 psl 抗性基因的黄瓜品系 Gy14 和易感 B10 品系的窄杂交开发了重组自交系 (RIL) 作图群体。在生长室条件下以及在田间测试了亲本系和 RIL 的角斑病症状。基于简单序列重复和DArTseq，构建了基因分型遗传图谱，包含7个连锁群717个位点，跨度599.7 cM，标记之间平均0.84 cM。病灶周围缺乏退绿晕的单基因遗传得到证实，这是 ALS 抗性的典型特征，并且与隐性 psl 抗性基因的存在有关。psl位点定位在黄瓜5号染色体上。发现与疾病严重程度相关的两个主要数量性状位点（QTL）psl5.1和psl5.2，并且位于5号染色体上，彼此相邻；此外，psl5.1 与 psl 基因座位于同一位置。鉴定出的QTL在田间试验中得到验证。构建的遗传图谱和与 ALS 抗性位点相关的标记是有助于黄瓜育种计划的新资源。