Adult plant resistance against plant pathogens is of interest as a means to achieve durable resistance. Prior to this research, the barley lines CLE210 (from Uruguay) and Denar (from the Czech Republic) had been reported to exhibit adult-plant resistance against powdery mildew. Here, populations of doubled haploid lines from crosses of these lines with the susceptible cultivar Baudin were evaluated for powdery mildew resistance in field experiments. Using linkage maps constructed from genotyping-by-sequencing (GBS) data, it was determined that differences in resistance were largely attributable to a region on the long arm of chromosome 5H (5HL). Therefore, KASP™ assays were developed based on GBS tag sequences mapped on that chromosome, providing more reliable genetic maps. In each population, a large-effect QTL was mapped on 5HL. As no sequence variation was detected between CLE210 and Denar in this region of 5HL, the two sources of resistance may be identical by descent in the QTL region and carry the same resistance gene. Marker assays from the QTL region were evaluated on a panel of barley lines, providing information that breeders could use to select assays for use in marker-assisted selection.

中文翻译：

---

大麦5H染色体上的一个位点影响成年植物对白粉病的抗性。

成年植物对植物病原体的抗性是获得持久抗性的一种手段。在进行这项研究之前，据报道，大麦系CLE210（来自乌拉圭）和Denar（来自捷克共和国）显示出成年植物对白粉病的抗性。在这里，在田间试验中，评估了从这些品系与易感品种博丁的杂交中获得的双倍单倍体品系群体的白粉病抗性。使用通过测序基因分型（GBS）数据构建的连锁图，可以确定抗性差异主要归因于5H染色体（5HL）长臂上的区域。因此，基于在该染色体上定位的GBS标签序列开发了KASP™分析，从而提供了更可靠的遗传图谱。在每个人群中，在5HL上均绘制了一个大的QTL。由于在5HL的该区域中CLE210和Denar之间未检测到序列变异，因此两个耐药源可能因QTL区域的下降而相同，并带有相同的耐药基因。在一组大麦系上评估了来自QTL区域的标记分析，提供了育种者可以用来选择用于标记辅助选择的分析的信息。