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Evaluation of two collagen conduits and autograft in rabbit sciatic nerve regeneration with quantitative magnetic resonance DTI, electrophysiology, and histology.
European Radiology Experimental ( IF 3.7 ) Pub Date : 2018-08-08 , DOI: 10.1186/s41747-018-0049-2
Tina Jeon 1 , Emil S Vutescu 2 , Eliana B Saltzman 2 , Jordan C Villa 2 , Scott W Wolfe 2 , Steve K Lee 2 , Joseph H Feinberg 3 , Sarah L Pownder 1 , Jonathan P Dyke 4 , Darryl B Sneag 1
Affiliation  

Background

We compared different surgical techniques for nerve regeneration in a rabbit sciatic nerve gap model using magnetic resonance diffusion tensor imaging (DTI), electrophysiology, limb function, and histology.

Methods

A total of 24 male New Zealand white rabbits were randomized into three groups: autograft (n = 8), hollow conduit (n = 8), and collagen-filled conduit (n = 8). A 10-mm segment of the rabbit proximal sciatic nerve was cut, and autograft or collagen conduit was used to bridge the gap. DTI on a 3-T system was performed preoperatively and 13 weeks after surgery using the contralateral, nonoperated nerve as a control.

Results

Overall, autograft performed better compared with both conduit groups. Differences in axonal diameter were significant (autograft > hollow conduit > collagen-filled conduit) at 13 weeks (autograft vs. hollow conduit, p = 0.001, and hollow conduit vs. collagen-filled conduit, p < 0.001). Significant group differences were found for axial diffusivity but not for any of the other DTI metrics (autograft > hollow conduit > collagen-filled conduit) (autograft vs. hollow conduit, p = 0.001 and hollow conduit vs. collagen-filled conduit, p = 0.021). As compared with hollow conduit (autograft > collagen-filled conduit > hollow conduit), collagen-filled conduit animals demonstrated a nonsignificant increased maximum tetanic force.

Conclusions

Autograft-treated rabbits demonstrated improved sciatic nerve regeneration compared with collagen-filled and hollow conduits as assessed by histologic, functional, and DTI parameters at 13 weeks.


中文翻译:

用定量磁共振 DTI、电生理学和组织学评估兔坐骨神经再生中的两种胶原蛋白导管和自体移植物。

背景

我们使用磁共振扩散张量成像 (DTI)、电生理学、肢体功能和组织学比较了兔坐骨神经间隙模型中神经再生的不同手术技术。

方法

将24只雄性新西兰白兔随机分为三组:自体移植(n  = 8)、空心导管(n  = 8)和胶原填充导管(n  = 8)。切断兔近端坐骨神经的 10 mm 段,并使用自体移植物或胶原导管弥合间隙。术前和术后 13 周使用对侧未手术神经作为对照,在 3-T 系统上进行 DTI。

结果

总体而言,与两个导管组相比,自体移植的表现更好。在 13 周时,轴突直径的差异显着(自体移植物 > 中空导管 > 胶原填充导管)(自体移植与中空导管,p  = 0.001,中空导管与胶原填充导管,p  < 0.001)。发现轴向扩散率的显着组差异,但没有发现任何其他 DTI 指标(自体移植 > 中空导管 > 胶原填充导管)(自体移植与中空导管,p  = 0.001 和中空导管与胶原填充导管,p  = 0.021)。与空心导管(自体移植 > 胶原填充导管 > 空心导管)相比,胶原填充导管动物表现出不显着的最大强直力增加。

结论

在 13 周时通过组织学、功能和 DTI 参数评估,与填充胶原蛋白和空心导管相比,自体移植治疗的兔子的坐骨神经再生得到改善。
更新日期:2018-08-08
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