Cowpea is one of the most important crops in West Africa and is essential for the region's food and nutrition security and economic development. Consequently, improving its agronomic performance and yield is a desirable goal. Brown blotch disease, caused by the fungal pathogen Colletotrichum capsici, is an important constraint of cowpea productivity, and at present, only limited genetic resources are available for breeding improved brown blotch-resistant varieties. The current study has characterized the genetic basis for brown blotch resistance conferred by the cowpea cultivar KN1 and identified a major dominant quantitative trait locus (QTL) for resistance on chromosome Vu02. A segregating F2 population (n = 200), derived from a cross between KN1 and brown blotch-susceptible Tiligre (KVx775-33-2G), was developed and scored for disease severity following controlled inoculation. A subset of the population (n = 94) was genotyped with 99 newly developed allele-specific polymerase chain reaction (AS-PCR) markers, and multiple interval mapping was performed. One major and three minor QTL were identified. This is the first reported mapping of QTL conferring resistance to C. capsici in cowpea, and it is expected that the markers identified here will be a valuable resource for developing elite cowpea cultivars with resistance to brown blotch.

中文翻译：

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cow豆品种KN1褐斑病抗性的定量性状基因座分析。

pea豆是西非最重要的农作物之一，对该地区的粮食和营养安全以及经济发展至关重要。因此，改善其农学性能和产量是理想的目标。真菌病原体炭疽菌引起的褐斑病是of豆生产力的重要制约因素，目前，只有有限的遗传资源可用于育种改良的褐斑病抗性品种。当前的研究已表征了the豆品种KN1赋予的棕色斑点抗性的遗传基础，并鉴定了Vu02染色体抗性的主要优势数量性状基因座（QTL）。分离的F2种群（n = 200），源自KN1和易感褐色斑点的蒂利格（KVx775-33-2G）之间的杂交，进行控制接种后，对疾病的严重程度进行评分并评分。使用99个新开发的等位基因特异性聚合酶链反应（AS-PCR）标记对人群的一部分（n = 94）进行基因分型，并进行了多区间作图。确定了一个主要和三个次要QTL。这是第一个报道的赋予cow豆对辣椒衣原体抗性的QTL定位图，预期在此鉴定的标记将是开发对褐斑病具有抗性的优良cow豆栽培种的宝贵资源。