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Anaerobic degradation of 2,4-dichlorophenoxyacetic acid by Thauera sp. DKT.
Biodegradation ( IF 3.6 ) Pub Date : 2018-08-13 , DOI: 10.1007/s10532-018-9848-7
Duc Danh Ha 1
Affiliation  

Thauera sp. strain DKT isolated from sediment utilized 2,4-dichlorophenoxyacetic acid (2,4D) and its relative compounds as sole carbon and energy sources under anaerobic conditions and used nitrate as an electron acceptor. The determination of 2,4D utilization at different concentrations showed that the utilization curve fitted well with the Edward model with the maximum degradation rate as 0.017 ± 0.002 mM/day. The supplementation of cosubstrates (glucose, acetate, sucrose, humate and succinate) increased the degradation rates of all tested chemical substrates in both liquid and sediment slurry media. Thauera sp. strain DKT transformed 2,4D to 2,4-dichlorophenol (2,4DCP) through reductive side-chain removal then dechlorinated 2,4DCP to 2-chlorophenol (2CP), 4-chlorophenol (4CP) and phenol before complete degradation. The relative degradation rates by the isolate in liquid media were: phenol > 2,4DCP > 2CP > 4CP > 2,4D ≈ 3CP. DKT augmentation in sediment slurry enhanced the degradation rates of 2,4D and chlorophenols. The anaerobic degradation rates in the slurry were significantly slower compared to the rates in liquid media.

中文翻译:

Thauera sp。厌氧降解2,4-二氯苯氧基乙酸。DKT。

Thauera sp。从沉积物中分离出的DKT菌株利用2,4-二氯苯氧基乙酸(2,4D)及其相关化合物作为厌氧条件下的唯一碳源和能源,并使用硝酸盐作为电子受体。在不同浓度下对2,4D利用率的测定表明,利用率曲线与Edward模型非常吻合,最大降解速率为0.017±0.002 mM / day。共底物(葡萄糖,乙酸盐,蔗糖,腐殖酸盐和琥珀酸盐)的添加增加了所有测试的化学底物在液体和沉积物淤浆介质中的降解率。陶拉sp。菌株DKT通过还原性侧链去除将2,4D转化为2,4-二氯苯酚(2,4DCP),然后将2,4DCP脱氯为2-氯苯酚(2CP),4-氯苯酚(4CP)和苯酚,然后完全降解。分离物在液体介质中的相对降解速率为:苯酚> 2,4DCP> 2CP> 4CP> 2,4D≈3CP。沉积物中淤浆的DKT增强提高了2,4D和氯酚的降解率。与液体介质中的速度相比,浆料中的厌氧降解速度要慢得多。
更新日期:2018-08-13
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