Milk oligosaccharides (OS) are bioactive molecules that impart a variety of health benefits to the consumer. Techniques commonly used to analyse and quantify OS require optimised extraction methods to separate the OS from more abundant milk components. Solid phase extraction (SPE) is frequently used to isolate milk OS from lactose; however, the literature contains no formal studies on its efficacy in this application. In this study, established SPE conditions were modified to improve the technique's effectiveness in purifying OS from lactose. Low concentrations of acetonitrile (ACN) and trifluoroacetic acid (TFA) were tested for solid phase washing. Lactose removal and retention of many OS were significantly improved when using 4% ACN/0.1% TFA compared with the more common water washing technique. Different behaviours between acidic and neutral OS were evident. The new SPE technique improves extraction efficiency for bovine milk OS in applications that do not require prior lactose hydrolysis.

中文翻译：

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一种石墨化碳固相萃取纯化牛奶低聚糖的改进方法

牛奶低聚糖 (OS) 是一种生物活性分子，可为消费者带来多种健康益处。常用于分析和量化 OS 的技术需要优化的提取方法，以将 OS 与更丰富的牛奶成分分离。固相萃取 (SPE) 常用于从乳糖中分离牛奶 OS；然而，文献中没有关于其在该应用中的功效的正式研究。在本研究中，对已建立的 SPE 条件进行了修改，以提高该技术从乳糖中纯化 OS 的有效性。测试了低浓度乙腈 (ACN) 和三氟乙酸 (TFA) 的固相洗涤。与更常见的水洗技术相比，当使用 4% ACN/0.1% TFA 时，许多 OS 的乳糖去除和保留得到显着改善。酸性和中性 OS 之间的不同行为很明显。在不需要预先乳糖水解的应用中，新的 SPE 技术提高了牛乳 OS 的提取效率。