BACKGROUND Articular cartilage diseases are considered a major health problem, and tissue engineering using human mesenchymal stem cells (MSCs) have been shown as a promising solution for cartilage tissue repair. Hesperidin is a flavonoid extract from citrus fruits with anti-inflammatory properties. We aimed to investigate the effect of hesperidin on MSCs for cartilage tissue repair. MSCs were treated by hesperidin, and colony formation and proliferation assays were performed to evaluate self-renewal ability of MSCs. Alcian blue staining and Sox9 expression were measured to evaluate chondrogenesis of MSCs. Secretion of pro-inflammatory cytokines IFN-γ, IL-2, IL-4 and IL-10, and expression of nuclear factor kappa B (NF-κB) subunit p65 were also assessed. RESULTS Hesperidin improved self-renewal ability and chondrogenesis of MSCs, inhibited secretion of pro-inflammatory cytokines IFN-γ, IL-2, IL-4 and IL-10, and suppressed the expression of p65. Overexpression of p65 was able to reverse the hesperidin inhibited secretions of pro-inflammatory cytokines, and abolish the enhancing effect of hesperidin on chondrogenesis of MSCs. CONCLUSION Hesperidin could serve as a therapeutic agent to effectively enhance chondrogenesis of human MSCs by inhibiting inflammation to facilitate cartilage tissue repair.

中文翻译：

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橙皮苷的抗炎作用增强人间充质干细胞的软骨形成，用于软骨组织修复。

背景技术关节软骨疾病被认为是主要的健康问题，使用人间充质干细胞（MSCs）的组织工程已被证明是软骨组织修复的有希望的解决方案。橙皮苷是一种来自柑橘类水果的类黄酮提取物，具有抗炎特性。我们旨在研究橙皮苷对 MSCs 软骨组织修复的影响。用橙皮苷处理 MSCs，并进行集落形成和增殖试验以评估 MSCs 的自我更新能力。测量阿新蓝染色和 Sox9 表达以评估 MSC 的软骨形成。还评估了促炎细胞因子 IFN-γ、IL-2、IL-4 和 IL-10 的分泌，以及核因子 kappa B (NF-κB) 亚基 p65 的表达。结果 橙皮苷改善了 MSCs 的自我更新能力和软骨形成，抑制促炎细胞因子 IFN-γ、IL-2、IL-4 和 IL-10 的分泌，并抑制 p65 的表达。p65的过表达能够逆转橙皮苷抑制促炎细胞因子的分泌，并消除橙皮苷对MSCs软骨形成的增强作用。结论橙皮苷可作为治疗药物，通过抑制炎症促进软骨组织修复，有效增强人间充质干细胞的软骨形成。