The complimentary effects of a native rhizobia strain (SAMFIX 286), lime and single superphosphate (SSP) as components of ISFM were evaluated on the biomass, nodulation and N₂ fixation of cowpea (Vigna unguiculata L.). Lime was applied at the rate of 250 kg (Ca(OH)₂) ha⁻¹, while SSP was applied at 30 kg P ha⁻¹. The trial was carried out in a screen house with the treatments arranged in randomized complete block design. Results obtained show that the performance of SAMFIX 286 inoculated plants did not significantly (p < 0.05) differ from that of the un-inoculated treatment. Application of lime significantly increased root dry weight, shoot dry weight, nodule number and dry weight by 42.5%, 35.3%, 65.6% and 50%, respectively. Nodulation was significantly (p < 0.05) increased by SSP. The complimentary effect of lime with SSP significantly increased total shoot N concentration. Similarly, combined inoculation of SAMFIX 286 with lime and SSP increased N concentration by 31.9% and N derived from atmosphere (Ndfa) by 16.3% compared to the un-inoculated treatment. Inoculation of SAMFIX 286 with SSP was also effective on Ndfa by cowpea. It was concluded that lime and SSP were good combination with native rhizobia strain in improving cowpea nodulation and biological N₂ fixation.

中文翻译：

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受尼日利亚根瘤菌，石灰和磷的本地菌株影响，strain豆的性能。

评价了天然根瘤菌菌株（SAMFIX 286），石灰和单过磷酸钙（SSP）作为ISFM的补充作用，对cow豆（Vigna unguiculata L.）的生物量，结瘤和N ₂固定作用。石灰的施用量为250 kg（Ca（OH）₂）ha ^-1，而SSP的施用量为30 kg P ha ^-1。该试验是在一个筛选房中进行的，处理方式以随机完整区组设计的形式安排。获得的结果表明，接种SAMFIX 286的植株的性能不显着（p <0.05）与未接种的治疗有所不同。施用石灰显着增加了根干重，枝干重，根瘤数和干重，分别增加了42.5％，35.3％，65.6％和50％。 SSP显着增加了结瘤（p <0.05）。石灰与SSP的互补作用显着增加了总苗N浓度。类似地，与未接种的处理相比，用石灰和SSP联合接种SAMFIX 286可使氮浓度增加31.9％，而源自大气的氮（Ndfa）则增加16.3％。cow豆对SAMFIX 286的SSP接种也对Ndfa有效。得出的结论是石灰和SSP用在改善豇豆结瘤和生物Ñ天然根瘤菌菌株良好组合₂固定。