DNA methylation plays an important role in the initiation and propagation of carcinogenesis; however, the role of heterogeneously methylated epialleles is currently not well studied, also due to the lack of sensitive, unbiased and high throughput methods. Here, a newly developed droplet digital PCR (ddPCR)-based method was evaluated regarding its ability to quantify such heterogeneously methylated epialleles with sufficient analytical sensitivity and specificity.

中文翻译：

---

使用等位基因敏感的液滴数字聚合酶链反应（EAST-ddPCR）鉴定和定量异甲基化的DNA片段。

DNA甲基化在致癌作用的起始和传播中起着重要作用。然而，由于缺乏灵敏，公正和高通量的方法，目前还没有很好地研究异质甲基化等位基因的作用。在这里，对一种新开发的基于液滴数字PCR（ddPCR）的方法进行了评估，该方法具有足够的分析灵敏度和特异性来量化这种异质甲基化的等位基因。