Advances in RNA sequencing technologies and computational methodologies have provided a huge impetus to noncoding RNA (ncRNA) study. Once regarded as inconsequential results of transcriptional promiscuity, ncRNAs were later found to exert great roles in various aspects of biological functions. They are emerging as key players in gene regulatory networks by interacting with other biomolecules (DNA, RNA or protein). Here, we provide an overview of ncRNA repertoire and highlight recent discoveries of their versatile interactions. To better investigate the ncRNA-mediated regulation, it is necessary to make full use of innovative sequencing techniques and computational tools. We further describe a comprehensive workflow for in silico ncRNA analysis, providing up-to-date platforms, databases and tools dedicated to ncRNA identification and functional annotation.

中文翻译：

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非编码RNA的多功能相互作用和生物信息学分析。

RNA测序技术和计算方法的进步为非编码RNA（ncRNA）研究提供了巨大动力。ncRNA一度被视为转录混杂的无关紧要的结果，后来发现在生物学功能的各个方面都发挥着重要作用。通过与其他生物分子（DNA，RNA或蛋白质）相互作用，它们正在成为基因调控网络中的关键角色。在这里，我们提供了ncRNA组成部分的概述，并着重介绍了其多功能相互作用的最新发现。为了更好地研究ncRNA介导的调控，有必要充分利用创新的测序技术和计算工具。我们进一步描述了用于计算机ncRNA分析的综合工作流程，提供了最新的平台，