In this study, samples were collected from the leaves and stems of healthy wild Pistachio trees (Pistacia atlantica L.) from various locations of Baneh and Marivan regions, Iran. In total, 61 endophytic bacteria were isolated and grouped according to phenotypic properties. Ten selected isolates from each group were further identified by partial sequencing of the 16S rRNA gene. Based on the results, isolates were identified as bacteria belonging to Pseudomonas, Stenotrophomonas, Bacillus, Pantoea and Serratia genus. The ability of these isolates was evaluated to phytohormone production such as auxin and gibberellin, siderophore production, phosphate solubilization, atmospheric nitrogen fixation, protease and hydrogen cyanide production. All strains were able to produce the plant growth hormone auxin and gibberellin in different amounts. The majority of strains were able to solubilize phosphate. The results of atmospheric nitrogen fixation ability, protease and siderophore production were varied among strains. Only Ba66 could produce a low amount of hydrogen cyanide. The results of biocontrol assay showed that Pb78 and Sp15 strains had the highest and lowest inhibition effects on bacterial plant pathogens, Pseudomonas syringae pv. syringae Pss20 and Pseudomonas tolaasii Pt18 under in vitro condition. Pb3, Pb24 and Pb71 strains significantly promote root formation on carrot slices. To our knowledge this is the first report of the isolation of endophytic bacterial strains belonging to Pantoea, Bacillus, Pseudomonas, Serratia and Stenotrophomonas genus from wild pistachio trees with plant growth promoting potential and biocontrol activity.

中文翻译：

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野生开心果树中具有植物生长促进活性和生物防治潜力的内生细菌的分离和鉴定。

在这项研究中，样本是从伊朗巴内和马里万地区不同地点的健康野生开心果树（ Pistacia atlantica L.）的叶子和茎中采集的。总共分离出 61 种内生细菌，并根据表型特性进行分组。通过 16S rRNA 基因的部分测序进一步鉴定了每组中的 10 个分离株。根据结果​​，分离菌株被鉴定为属于假单胞菌属、寡养单胞菌属、芽孢杆菌属、泛菌属和沙雷氏菌属的细菌。评估了这些分离株的植物激素生产（例如生长素和赤霉素）、铁载体生产、磷酸盐溶解、大气固氮、蛋白酶和氰化氢生产的能力。所有菌株都能产生不同量的植物生长激素生长素和赤霉素。大多数菌株能够溶解磷酸盐。不同菌株的大气固氮能力、蛋白酶和铁载体产量结果存在差异。只有Ba66可以产生少量的氰化氢。生防试验结果表明，Pb78和Sp15菌株对细菌性植物病原菌丁香假单胞菌（Pseudomonas syringae pv .）的抑制效果最高和最低。体外条件下的丁香菌Pss20 和假单胞菌Pt18 。Pb3、Pb24 和 Pb71 菌株显着促进胡萝卜片上根的形成。据我们所知，这是首次从野生开心果树中分离出属于泛菌属、芽孢杆菌属、假单胞菌属、沙雷氏菌属和寡养单胞菌属的内生细菌菌株，这些菌株具有促进植物生长的潜力和生物防治活性。