Fruit rot is the principal phytopathological problem of pipiana pumpkin (Cucurbita argyrosperma Huber) in the state of Guerrero. The aims of this research were to 1) identify the causal agent of southern blight on pumpkin fruits by morphological, pathogenic, and molecular analysis (ITS1, 5.8S, ITS2); 2) evaluate in vitro Trichoderma spp. strains and chemical fungicides; and 3) evaluate under rainfed field conditions, the strains that obtained the best results in vitro, combined with fungicides during two crop cycles. Number of commercial and non-commercial fruits at harvest, and seed yield (kg ha^-1) were registered. Morphological, pathogenic and molecular characterization identified Sclerotium rolfsii as the causal agent of rot in pipiana pumpkin fruits. Now, in vitro conditions, the highest inhibition of S. rolfsii were obtained by Trichoderma virens strain G-41 (70.72%), T. asperellum strain CSAEGro-1 (69%), and the fungicides metalaxyl (100%), pyraclostrobin (100%), quintozene (100%), cyprodinil + fludioxonil (100%), and prochloraz (100%). Thiophanate-methyl only delayed growth (4.17%). In field conditions, during the spring-summer 2015 cycle, T. asperellum strain CSAEGro-1 + metalaxyl, and T. asperellum + cyprodinil + fludioxonil, favored the highest number of fruits and seed yield in the crop.

中文翻译：

---

银核菌引起的银瓜果实腐烂病的诊断与综合治理。


果实腐烂是格雷罗州 pipiana 南瓜 ( Cucurbita argyrosperma Huber) 的主要植物病理学问题。本研究的目的是 1) 通过形态学、病原学和分子分析（ITS1、5.8S、ITS2）确定南瓜果实上白疫病的致病因子； 2)体外评估木霉属物种。菌株和化学杀菌剂； 3) 在雨养田间条件下评估在两个作物周期中与杀菌剂结合使用时在体外获得最佳效果的菌株。记录收获时商业和非商业水果的数量以及种子产量（kg ha ^-1 ）。形态学、病原学和分子特征鉴定表明， Sclerotium rolfsii是导致 pipiana 南瓜果实腐烂的病原体。目前，在体外条件下，对罗氏沙门氏菌的抑制作用最高的是木霉菌株 G-41 (70.72%)、 T. asperellum菌株 CSAEGro-1 (69%) 以及杀菌剂甲霜灵 (100%)、唑菌胺酯 (100%)。 100%）、五氯硝基苯（100%）、嘧菌环胺+咯菌腈（100%）和咪鲜胺（100%）。甲基托布津仅延迟生长（4.17%）。在田间条件下，在 2015 年春夏周期期间， T. asperellum菌株 CSAEGro-1 + 甲霜灵，以及T. asperellum + 环菌环胺 + 咯菌腈，有利于作物中最高数量的果实和种子产量。