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Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees.
The Plant Pathology Journal ( IF 1.8 ) Pub Date : 2018-06-01 , DOI: 10.5423/ppj.oa.09.2017.0194
Camila Vilela Vasconcelos 1 , Fabíola Teodoro Pereira 1 , Elizabeth Amélia Alves Duarte 2 , Thiago Alves Santos de Oliveira 3 , Nei Peixoto 1 , Daniel Diego Costa Carvalho 1
Affiliation  

The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

中文翻译:

芒果树中的头孢霉菌的生理和分子表征。

这项工作的目的是完成芒果树藻斑病病原菌的分离,分子鉴定和生理学表征。为此目的,在不同的培养基中评估病原体的生长,随后观察和测量丝状细胞。分子鉴定是使用从叶片损伤和在培养基中生长的纯藻类菌落获得的菌丝体进行的。基于DNA测序的描述表明藻类是Cephaleuros virescens。必须将藻类主要分离在液体培养基中,以便进一步刺入琼脂培养基。当藻类在Trebouxia和BBM中生长时,发生在陪替氏培养皿中的最高菌丝生长平均值。与其他培养基相比,Trebouxia使丝状细胞中的细胞更大。
更新日期:2020-08-21
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