Background Kinesin superfamily proteins are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. However, their exact roles during mammalian oocyte meiosis have not been fully clarified. Results Herein, we investigated the critical events during porcine oocyte meiotic maturation with the treatment of Eg5-specific inhibitor monastrol. We found that Eg5 inhibition resulted in oocyte meiotic failure by displaying the poor expansion of cumulus cells and reduced rate of polar body extrusion. In the meantime, the spindle assembly and chromosome alignment were compromised, accompanied by the decreased level of acetylated α-tubulin, indicative of less stable microtubules. Impaired actin dynamics and mitochondria integrity were also observed in Eg5-inhibited oocytes. Additionally, inhibition of Eg5 caused the abnormal distribution of cortical granules and ovastacin, a cortical granule component, potentially leading to the fertilization failure. Conclusions Our findings reveal that Eg5 possesses an important function in porcine oocyte meiotic progression by regulating the organelle dynamics and arrangement.

中文翻译：

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Eg5 通过维持减数分裂细胞器排列来协调猪卵母细胞的成熟进程。

背景 驱动蛋白超家族蛋白是基于微管的分子马达，对于包括细胞器、蛋白质和 RNA 在内的各种货物的细胞内运输至关重要。然而，它们在哺乳动物卵母细胞减数分裂中的确切作用尚未完全阐明。结果在此，我们研究了在猪卵母细胞减数分裂成熟期间用 Eg5 特异性抑制剂 monastrol 治疗的关键事件。我们发现 Eg5 抑制通过显示卵丘细胞的不良扩张和极体挤出率降低而导致卵母细胞减数分裂失败。与此同时，纺锤体组装和染色体排列受到损害，伴随着乙酰化α-微管蛋白水平的降低，表明微管不太稳定。在 Eg5 抑制的卵母细胞中也观察到肌动蛋白动力学和线粒体完整性受损。此外，抑制 Eg5 导致皮质颗粒和 ovastacin（一种皮质颗粒成分）分布异常，可能导致受精失败。结论 我们的研究结果表明，Eg5 通过调节细胞器动力学和排列在猪卵母细胞减数分裂进程中具有重要作用。