BACKGROUND Previous studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process. METHODS 2D-PAGE, NanoLC-MS/MS with higher resolution, Ensembl lamprey and NCBI protein databases, as well as western blot was used to compare the proteomics of buccal gland secretion from China northeast lampreys (Lampetra morii) which had been fed for 0, 10, and 60 min, respectively. RESULTS In the present study, the number of identified protein species in the buccal glands of feeding groups (60 min) was increased significantly, nearly ten times of that in the fasting group. During the feeding stage, novel proteins emerged in the buccal gland secretion of lampreys. According to gene ontology (GO) analysis and function predictions, these proteins were summarized and discussed based on their potential roles during feeding process. Furthermore, some of the identified proteins were confirmed to express during the feeding time of lampreys. CONCLUSION When lampreys attack host fishes to suck blood and flesh, their buccal glands could secrete enough proteins to suppress blood coagulation, nociception, oxidative stress, immune response, as well as other adverse effects encountered during their parasitic lives. The present study would provide clues to clarify the feeding mechanism of the bloodsucking lampreys.

中文翻译：

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禁食和喂养七鳃鳗（Lampetra morii）颊腺分泌物的蛋白质组学分析。

背景技术以往的研究表明，七鳃鳗颊腺由于吸血习惯而含有一些与抗凝、伤害感受和免疫反应相关的调节剂。遗憾的是，从未报道过喂养七鳃鳗颊腺中的蛋白质表达谱。本研究旨在进一步鉴定更多与七鳃鳗摄食过程密切相关的蛋白质。方、 10 和 60 分钟，分别。结果 在本研究中，进食组（60 min）颊腺中鉴定出的蛋白质种类数量显着增加，是禁食组的近10倍。在摄食阶段，七鳃鳗的颊腺分泌物中出现了新的蛋白质。根据基因本体（GO）分析和功能预测，对这些蛋白质在摄食过程中的潜在作用进行了总结和讨论。此外，一些已鉴定的蛋白质被证实在七鳃鳗的摄食期间表达。结论七鳃鳗攻击寄主鱼类吸血和吸肉时，其颊腺可以分泌足够的蛋白质来抑制血液凝固、伤害感受、氧化应激、免疫反应以及寄生生活中遇到的其他不利影响。