The aims of the study were to evaluate the effect of media composition on the growth potential and morphology of the in vitro cultured biomass of three cultivars of Hypericum perforatum, and on the production of flavonoids. Agitated shoot cultures were maintained in parallel on Linsmaier and Skoog (LS) and Murashige and Skoog (MS) media supplemented with 0.1-3.0 mg L-1 of α-naphthaleneacetic acid and 6-benzylaminopurine. Methanolic extracts from the biomass collected after 3-wk growth cycles were analyzed quantitatively, for 21 flavonoids using high performance liquid chromatography. Three aglycones (kaempferol, luteolin, and quercetin) and three glycosides of quercetin (hyperoside, quercitrin, and rutoside) were detected in all of the extracts. The total amounts of the estimated compounds increased from 1.18- to 21.66-fold on LS media variants and from 1.52- to 17.34-fold on MS media variants. The main metabolite was quercetin (max. 210.55 mg 100 g-1 dry weight [DW]). The maximum total amounts of all compounds in the biomass of 'Elixir,' 'Helos,' and 'Topas' were 328.53, 255.70, and 166.58 mg 100 g-1 DW, respectively. The shoots of all cultivars cultivated on the LS and MS media containing low levels of plant growth regulators (0.1 mg L-1) accumulated high amounts of flavonoids. The highest amounts were accumulated in shoots of cultivar 'Elixir' grown on MS medium. This is the first comparison of flavonoid production in three cultivars of H. perforatum ('Elixir,' 'Helos,' and 'Topas') cultured in vitro, and the first report of flavonoid production in cultivars 'Elixir' and 'Helos.'

中文翻译：

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培养基组成对三个贯叶金丝桃栽培品种'长生不老药'，'Helos'和'Topas'的搅拌芽培养中类黄酮产生的影响。

该研究的目的是评估培养基组成对三个贯叶金丝桃的体外培养生物量的生长潜力和形态以及对类黄酮产生的影响。在Linsmaier和Skoog（LS）以及Murashige和Skoog（MS）培养基上，同时补充0.1-3.0 mg L-1的α-萘乙酸和6-苄基氨基嘌呤，同时维持搅动的芽培养物。使用高效液相色谱法对3周生长周期后收集的生物质中的甲醇提取物进行了定量分析，确定了21种类黄酮。在所有提取物中均检出了三种苷元（山奈酚，木犀草素和槲皮素）和三种槲皮素糖苷（hyperoside，槲皮素和芸香苷）。估计化合物的总量从1.18-增加到21。在LS培养基变体上是66倍，在MS培养基变体上是1.52到17.34倍。主要代谢物是槲皮素（最大210.55 mg 100 g-1干重[DW]）。“ Elixir”，“ Helos”和“ Topas”生物量中所有化合物的最大总量分别为328.53、255.70和166.58 mg 100 g-1 DW。在含有低水平植物生长调节剂（0.1 mg L-1）的LS和MS培养基上培养的所有品种的芽均积累了大量的类黄酮。在MS培养基上生长的品种'Elixir'的芽中积累的量最高。这是体外培养的三个贯叶连翘（'Elixir'，'Helos'和'Topas'）黄酮类化合物产量的首次比较，也是关于'Elixir'和'Helos'品种黄酮类化合物产量的首次报道。