A set of 178 Escherichia coli isolates taken from patients with inflammatory bowel disease (IBD) was analyzed for bacteriocin production and tested for the prevalence of 30 bacteriocin and 22 virulence factor determinants. Additionally, E. coli phylogenetic groups were also determined. Pulsed-field gel electrophoresis (PFGE) was used for exclusion of clonal character of isolates. Results were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. The frequency of bacteriocinogenic isolates (66.9%) was significantly higher in IBD E. coli compared to fecal commensal E. coli isolates (54.2%, p < 0.01). In the group of IBD E. coli isolates, a higher prevalence of determinants for group B colicins (i.e., colicins B, D, Ia, Ib, M, and 5/10) (p < 0.01), including a higher prevalence of the colicin B determinant (p < 0.01) was found. Virulence factor determinants encoding fimbriae (fimA, 91.0%; pap, 27.5%), cytotoxic necrotizing factor (cnf1, 11.2%), aerobactin synthesis (aer, 43.3%), and the locus associated with invasivity (ial, 9.0%) were more prevalent in IBD E. coli (p < 0.05 for all five determinants). E. coli isolates from IBD mucosal biopsies were more frequently bacteriocinogenic (84.6%, p < 0.01) compared to fecal IBD isolates and fecal commensal E. coli. PFGE analysis revealed clusters specific for IBD E. coli isolates (n = 11), for fecal isolates (n = 13), and clusters containing both IBD and fecal isolates (n = 10). ExPEC (Extraintestinal Pathogenic E. coli) virulence and colicin determinants appear to be important characteristics of IBD E. coli isolates, especially the E. coli isolates obtained directly from biopsy samples.

中文翻译：

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从炎症性肠病患者中分离出大肠杆菌：与健康对照组相比，ExPEC毒力和大肠菌素决定簇更为频繁。

分析了一组来自炎症性肠病（IBD）患者的178株大肠杆菌菌株的细菌素产生量，并测试了30种细菌素和22种毒力因子决定因素的发生率。此外，还确定了大肠杆菌的系统发生群。脉冲场凝胶电泳（PFGE）用于排除分离株的克隆特征。将结果与先前发布的1283个粪便共生大肠杆菌分离物的分析数据进行比较。与粪便型大肠杆菌分离株相比，IBD大肠杆菌中细菌致癌分离株的频率（66.9％）显着更高（54.2％，p <0.01）。在IBD大肠杆菌分离物组中，B组大肠菌素（即大肠菌素B，D，Ia，Ib，M和5/10）的决定因素的患病率较高（p <0.01），发现大肠菌素B决定簇的患病率较高（p <0.01）。编码菌毛的毒力因子决定因素（fimA，91.0％; pap，27.5％），细胞毒性坏死因子（cnf1，11.2％），气杆菌素合成（aer，43.3％），以及与侵袭性相关的基因座（ial，9.0％）在IBD大肠杆菌中普遍存在（对于所有五个决定因素，p <0.05）。与粪便IBD分离物和粪便共生大肠杆菌相比，IBD黏膜活检分离株更易致细菌（84.6％，p <0.01）。PFGE分析揭示了对IBD大肠杆菌分离株（n = 11），对粪便分离株（n = 13）和同时含有IBD和粪便分离株（n = 10）的簇。ExPEC（肠道外致病性大肠杆菌）毒力和大肠菌素决定因素似乎是IBD大肠杆菌分离物（尤其是大肠杆菌）的重要特征。