We report the synthesis, characterization, and assessment of a nanoparticle-based RNAi delivery platform that protects siRNA payloads against nuclease-induced degradation and efficiently delivers them to target cells. The nanocarrier is based on biodegradable mesoporous silicon nanoparticles (pSiNPs), where the voids of the nanoparticles are loaded with siRNA and the nanoparticles are encapsulated with graphene oxide nanosheets (GO-pSiNPs). The graphene oxide encapsulant delays release of the oligonucleotide payloads in vitro by a factor of 3. When conjugated to a targeting peptide derived from the rabies virus glycoprotein (RVG), the nanoparticles show 2-fold greater cellular uptake and gene silencing. Intravenous administration of the nanoparticles into brain-injured mice results in substantial accumulation specifically at the site of injury.

中文翻译：

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用于将 siRNA 靶向递送至受伤大脑的多孔硅-氧化石墨烯核壳纳米颗粒。

我们报告了一种基于纳米颗粒的 RNAi 递送平台的合成、表征和评估，该平台保护 siRNA 有效载荷免受核酸酶诱导的降解，并有效地将它们递送至靶细胞。纳米载体基于可生物降解的介孔硅纳米粒子 (pSiNPs)，其中纳米粒子的空隙装载有 siRNA，纳米粒子用氧化石墨烯纳米片 (GO-pSiNPs) 封装。氧化石墨烯封装剂在体外将寡核苷酸有效载荷的释放延迟了 3 倍。当与狂犬病病毒糖蛋白 (RVG) 衍生的靶向肽结合时，纳米颗粒显示出 2 倍的细胞摄取和基因沉默。将纳米粒子静脉注射到脑损伤小鼠体内会导致大量积聚，特别是在损伤部位。