BACKGROUND Transcription factors could regulate multiple aspects of plants growth and development, which is significant to plants. Ovate family proteins (OFPs) that are named due to contain OVATE domain, a 70-AA C-terminal conserved domain from the protein OVATE gene encodes, are plant-specific transcription factors family. Some members of OFPs have been shown to function as transcription factors to regulate plant growth and development, but little is known about the function of AtOFP8. RESULTS Here, we found AtOFP8 maybe involve in transcriptional regulation of the epicuticular waxes in Arabidopsis thaliana. First, we observed that the distribution of epicuticular waxes of wild type plants was more than that of Atofp8-1 deletion mutants, but less than that of 35S:HA-AtOFP8 transgenic overexpression lines not only on the leaves but also on the stems utilizing scanning electron microscopes. Second, we extracted waxes from leaves and stems of three types of plants respectively to measure the waxes content and composition by gas chromatography-mass spectrometer (GC-MS), and the results of the total content of waxes were consistent with the results of scanning electron microscopes. Finally, we found that the expression of 12 genes related to the synthesis and metabolism of waxes was changed in the Atofp8-1 mutants and 35S:HA-AtOFP8 transgenic lines compared with wild type plants. CONCLUSIONS These findings suggest that AtOFP8 could change the accumulation of epicuticular waxes.

中文翻译：

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卵形家族蛋白8的表达影响拟南芥中表皮蜡的积累。

背景技术转录因子可以调节植物生长和发育的多个方面，这对于植物而言是重要的。卵形家族蛋白（OFPs）是植物特异性转录因子家族，因其含有OVATE域而得名，OVATE域是蛋白OVATE基因编码的70-AA C端保守域。已经显示，OFP的某些成员起调节植物生长和发育的转录因子的作用，但对AtOFP8的功能知之甚少。结果在这里，我们发现AtOFP8可能参与拟南芥表皮蜡的转录调控。首先，我们观察到野生型植物的表皮蜡的分布大于Atofp8-1缺失突变体，但小于35S：利用扫描电子显微镜，HA-AtOFP8转基因过表达系不仅在叶片上，而且在茎上。其次，分别从三种植物的叶片和茎中提取蜡，用气相色谱-质谱仪（GC-MS）测量蜡的含量和组成，蜡的总含量与扫描结果相符。电子显微镜。最后，我们发现与野生型植物相比，Atofp8-1突变体和35S：HA-AtOFP8转基因株系中与蜡的合成和代谢相关的12个基因的表达发生了变化。结论这些发现表明AtOFP8可以改变表皮蜡的积累。我们分别从三种植物的叶片和茎中提取蜡，用气相色谱-质谱仪（GC-MS）测定蜡的含量和组成，蜡的总含量与扫描电子显微镜的结果一致。 。最后，我们发现与野生型植物相比，Atofp8-1突变体和35S：HA-AtOFP8转基因株系中与蜡的合成和代谢相关的12个基因的表达发生了变化。结论这些发现表明AtOFP8可以改变表皮蜡的积累。我们分别从三种植物的叶和茎中提取蜡，用气相色谱-质谱仪（GC-MS）测量蜡的含量和组成，蜡的总含量与扫描电子显微镜的结果一致。 。最后，我们发现与野生型植物相比，Atofp8-1突变体和35S：HA-AtOFP8转基因株系中与蜡的合成和代谢相关的12个基因的表达发生了变化。结论这些发现表明AtOFP8可以改变表皮蜡的积累。我们发现与野生型植物相比，Atofp8-1突变体和35S：HA-AtOFP8转基因系中与蜡的合成和代谢相关的12个基因的表达发生了变化。结论这些发现表明AtOFP8可以改变表皮蜡的积累。我们发现与野生型植物相比，Atofp8-1突变体和35S：HA-AtOFP8转基因系中与蜡的合成和代谢相关的12个基因的表达发生了变化。结论这些发现表明AtOFP8可以改变表皮蜡的积累。