Electron microscopy and X-ray diffraction have together played a key role in our understanding of the molecular structure and mechanism of contraction of muscle. This review highlights the role of electron microscopy, from early insights into thick and thin filament structure by negative staining, to studies of single myosin molecule structure, and finally to recent high-resolution structures by cryo-electron microscopy. Muscle filaments are designed for movement. Their labile structures thus present challenges to obtaining near-atomic detail, which are also discussed.

中文翻译：

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电子显微镜测定的肌丝分子结构

电子显微镜和 X 射线衍射在我们理解肌肉收缩的分子结构和机制方面发挥了关键作用。这篇综述强调了电子显微镜的作用，从早期通过负染色对粗细丝结构的了解，到单肌球蛋白分子结构的研究，最后到最近通过低温电子显微镜的高分辨率结构。肌丝专为运动而设计。因此，它们的不稳定结构对获得近原子细节提出了挑战，这也被讨论了。