This study compared osteoinductivity and osteogenic capacity between AB204 and rhBMP-2 using hMSCs in vitro and a beagle’s posterolateral spinal fusion model. Cultured hMSCs were treated with AB204 or rhBMP-2 with low to high doses. Three male beagles were performed posterolateral spinal fusion with biphasic calcium phosphate (2 ml) + AB204 or rhBMP-2 (20, 50 or 200 µg). They were euthanized after 8 weeks. The fusion rate and bone formation of spine samples were examined. AB204 had higher alkaline phosphatase activity, mineralization and osteogenic-related gene expression than rhBMP-2. Fusion rates in all rhBMP-2 groups were 0. They were 100% for 50 μg and 200 μg AB204 groups. Therefore, AB204 showed higher osteogenicity than rhBMP-2. It could be a better bone graft substitute.

这项研究比较了体外使用hMSCs和比格犬后外侧脊柱融合模型在AB204和rhBMP-2之间的骨诱导能力和成骨能力。用AB204或rhBMP-2低至高剂量处理培养的hMSC。将三只雄性比格犬与双相磷酸钙（2 ml）+ AB204或rhBMP-2（20、50或200 µg）进行后外侧脊柱融合。他们在8周后被安乐死。检查了脊柱样品的融合率和骨形成。与rhBMP-2相比，AB204具有更高的碱性磷酸酶活性，矿化作用和成骨相关基因的表达。所有rhBMP-2组的融合率均为0。对于50μg和200μgAB204组，融合率为100％。因此，AB204显示出比rhBMP-2更高的成骨性。它可能是更好的骨移植替代品。