This study investigates the significance of the expression and dynamics of podoplanin in mechanostress and mineralization in cultured murine osteoblasts. Podoplanin increased in osteoblasts subjected to straining in non-mineralization medium, suggesting that the mechanostress alone is a podoplanin induction factor. In osteoblasts subjected to vertical elongation straining in the mineralization medium, the mRNA amounts of podoplanin, osteopontin, and osteocalcin were significantly larger than those in cells not subjected to straining, suggesting that mechanostress is the cause of a synergistic effect in the expression of these proteins. In osteoblasts in the mineralization medium, significant increases in osteocalcin mRNA occurred earlier in cells subjected to straining than in the cells not subjected to straining, suggesting that the mechanostress is a critical factor to enhance the expression of osteocalcin. Western blot and ELISA analysis showed increased podoplanin production in osteoblasts with longer durations of straining. There was significantly less mineralization product in osteoblasts with antibodies for podoplanin, osteopontin, and osteocalcin. There was also less osteopontin and osteocalcin produced in osteoblasts with anti-podoplanin. These findings suggest that mechanostress induces the production of podoplanin in osteoblasts and that podoplanin may play a role in mineralization in cooperation with bone-associated proteins.

中文翻译：

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Podoplanin在具有机械应力和矿化刺激作用的成骨细胞中的表达和动力学。

这项研究调查了podoplanin的表达和动力学在培养的鼠成骨细胞的机械应力和矿化中的意义。在非矿化培养基中经受应变的成骨细胞中，podoplanin含量增加，表明仅机械应力是podoplanin诱导因子。在矿化培养基中进行垂直延伸拉紧的成骨细胞中，podoplanin，骨桥蛋白和骨钙素的mRNA量显着大于未拉紧细胞的mRNA量，这表明机械应力是这些蛋白表达协同作用的原因。 。在矿化培养基中的成骨细胞中，受过滤的细胞中骨钙素mRNA的发生比未受过滤的细胞中的骨钙素mRNA显着增加的时间早，这表明机械应力是增强骨钙素表达的关键因素。Western印迹和ELISA分析表明，成骨细胞的Podoplanin产量增加，且过滤时间更长。成骨细胞中含有足动蛋白，骨桥蛋白和骨钙素抗体的矿物质明显减少。用抗Podoplanin在成骨细胞中产生的骨桥蛋白和骨钙蛋白也较少。这些发现表明，机械应力诱导成骨细胞中Podoplanin的产生，并且Podoplanin与骨相关蛋白协同作用可能在矿化中发挥作用。成骨细胞中含有足动蛋白，骨桥蛋白和骨钙素抗体的矿物质明显减少。用抗Podoplanin在成骨细胞中产生的骨桥蛋白和骨钙蛋白也较少。这些发现表明，机械应力诱导成骨细胞中Podoplanin的产生，并且Podoplanin与骨相关蛋白协同作用可能在矿化中发挥作用。成骨细胞中含有足动蛋白，骨桥蛋白和骨钙素抗体的矿物质明显减少。用抗Podoplanin在成骨细胞中产生的骨桥蛋白和骨钙蛋白也较少。这些发现表明，机械应力诱导成骨细胞中Podoplanin的产生，并且Podoplanin与骨相关蛋白协同作用可能在矿化中发挥作用。