ABSTRACT The accessibility of selenium from naturally enriched sources such as cereals crops can potentially be used as selenium supplements to support nutritional requirements. Dietary selenium supplementation, as Se-rich wheat extracts, on RAW264.7 macrophage cells enhanced the antioxidant capacity via augmentation of cellular selenoprotein glutathione peroxidase 1 (GPx-1) expression in the absence or presence of lipopolysaccharide (LPS) treatment. Cells were supplemented with Se in the form of sodium selenite (SS), seleniferous wheat extract (SeW) and seleniferous wheat extract with rMETase treatment (SeW + rMET) at three different concentrations. Cells supplemented with SS and SeW + rMET showed increase in GPx-1 expression as compared to SeW-treated cells. SeW + rMET, further, downregulated the LPS-induced expression of cyclooxygenase-2, microsomal PGE synthase-1 and inducible nitric oxide synthase w.r.t. Se-deficient cells, while the expression of haematopoietic PGD synthase was upregulated. This demonstrates SeSup effectively modulates the expression of inflammatory responses, indicating the potential benefits of dietary selenium supplementation.

中文翻译：

---

通过富硒膳食基质补充硒可以上调脂多糖刺激的小鼠巨噬细胞的抗炎反应


摘要：从谷类作物等天然富集来源获取的硒有可能用作硒补充剂，以满足营养需求。在不存在或存在脂多糖 (LPS) 处理的情况下，通过增加细胞硒蛋白谷胱甘肽过氧化物酶 1 (GPx-1) 的表达，在 RAW264.7 巨噬细胞上补充富含硒的小麦提取物，从而增强抗氧化能力。细胞以三种不同浓度的亚硒酸钠 (SS)、含硒小麦提取物 (SeW) 和经 rMETase 处理的含硒小麦提取物 (SeW + rMET) 形式补充硒。与 SeW 处理的细胞相比，补充 SS 和 SeW + rMET 的细胞显示 GPx-1 表达增加。 SeW + rMET 进一步下调 LPS 诱导的缺硒细胞中环氧合酶 2、微粒体 PGE 合酶 1 和诱导型一氧化氮合酶的表达，同时上调造血 PGD 合酶的表达。这表明 SeSup 可以有效调节炎症反应的表达，表明膳食补硒的潜在益处。