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Out-of-focal plane imaging by leakage radiation microscopy
Journal of Optics ( IF 2.0 ) Pub Date : 2017-08-09 , DOI: 10.1088/2040-8986/aa79cc
Liangfu Zhu 1 , Douguo Zhang 1 , Ruxue Wang 1 , Xiaolei Wen 2 , Pei Wang 1 , Hai Ming 1 , Ramachandram Badugu 3 , Joseph R Lakowicz 3
Affiliation  

Leakage radiation microscopy (LRM) is used to investigate the optical properties of surfaces. The front-focal plane (FFP) image with LRM reveals structural features on the surfaces. Back-focal plane (BFP) image with LRM reveals the angular distribution of the radiation. Herein we experimentally demonstrate that the out-of-focal plane (OFP) images present a link between the FFP and BFP images and provide optical information that cannot be resolved by either FFP or BFP images. The OFP image provides a linkage between the spatial location of the emission and the angular distribution from the same location, and thus information about the film's discontinuity, nonuniformity or variable thickness can be uncovered. The use of OFP imaging will extend the scope and applications of the LRM and coupled emission imaging which are powerful tools in nanophotonics and high throughput fluorescence screening.

中文翻译:

通过泄漏辐射显微镜进行焦平面外成像

泄漏辐射显微镜 (LRM) 用于研究表面的光学特性。带有 LRM 的前焦平面 (FFP) 图像揭示了表面的结构特征。具有 LRM 的后焦平面 (BFP) 图像揭示了辐射的角度分布。在这里,我们通过实验证明了焦平面外 (OFP) 图像呈现了 FFP 和 BFP 图像之间的联系,并提供了 FFP 或 BFP 图像无法解析的光学信息。OFP 图像提供了发射的空间位置和同一位置的角度分布之间的联系,因此可以揭示有关薄膜不连续性、不均匀性或可变厚度的信息。
更新日期:2017-08-09
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