The pore forming characteristic of TDH1 and TDH2 variants of thermostable direct hemolysin (TDH), a major toxin involved in the pathogenesis of Vibrio parahaemolyticus, was studied on a planar lipid bilayer painted over individual picoliter cavities containing microelectrodes assembled in a multiarray. Both proteins formed pores upon insertion into the lipid bilayer which was shown as a shift in the conductance from the baseline current. TDH2 protein was able to produce stable currents and the currents were influenced by external factors like concentration, type of salt and voltage. The pore currents were influenced and showed a detectable response in the presence of polymers which makes them suitable for biotechnology applications.

中文翻译：

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无细胞生产成孔毒素：来自副溶血性弧菌的热稳定直接溶血素的功能分析

热稳定直接溶血素 (TDH) 是一种参与副溶血性弧菌发病机制的主要毒素，TDH1 和 TDH2 变体的成孔特征在涂在单个皮升腔上的平面脂质双层上进行了研究，该腔包含组装在多阵列中的微电极。两种蛋白质都在插入脂质双层后形成孔，这显示为电导从基线电流的变化。TDH2蛋白能够产生稳定的电流，并且电流受到浓度、盐的类型和电压等外部因素的影响。孔电流受到影响并在聚合物存在下显示出可检测的响应，这使得它们适用于生物技术应用。