Attenuated total reflectance-Fourier transform infrared (FTIR-ATR) spectroscopy was applied to 120 samples of heartwood rings from eight individual pine trees from different locations in Spain. Pinus sylvestris cores were collected at the Artikutza natural park (Ps-ART). Pinus nigra cores were collected in Sierra de Cazorla (Pn-LIN) and in La Sagra Mountain (Pn-LSA). Three discriminant analysis tests were performed using all bands (DFT), lignin bands only (DFL) and polysaccharides bands only (DFP), to explore the ability of FTIR-ATR to separate between species and growing location. The DFL model enabled a good separation between pine species, whereas the DFP model enabled differentiation for both species and growing location. The DFT model enabled virtually perfect separation, based on two functions involving twelve FTIR bands. Discrimination between species was related to bands at 860 and 1655 cm−1, which were more intense in P. sylvestris samples, and bands at 1425 and 1635 cm−1, more intense in P. nigra samples. These vibrations were related to differences in lignin structure and polysaccharide linear chains. Discrimination between growing locations was mainly related to polysaccharide absorptions: at 900, 1085 and 1335 cm−1 more representative of Pn-LIN samples, and at 1105 and 1315 cm−1 mostly associated to Pn-LSA samples. These absorptions are related to β-glycosidic linkages (900 cm−1), cellulose and hemicellulose (C–O bonds, 1085 and 1105 cm−1) and content in amorphous/crystalline cellulose (1315 and 1335 cm−1). These results show that FTIR-ATR in combination with multivariate statistics can be a useful tool for species identification and provenancing for pine wood samples of unknown origin.

中文翻译：

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使用 FTIR-ATR 根据树种和生长地点区分松木

衰减全反射傅里叶变换红外 (FTIR-ATR) 光谱应用于来自西班牙不同地点的八棵松树的 120 个心材年轮样品。在 Artikutza 自然公园 (Ps-ART) 收集了樟子松核。在 Sierra de Cazorla (Pn-LIN) 和 La Sagra Mountain (Pn-LSA) 中收集了黑松核。使用所有条带 (DFT)、仅木质素条带 (DFL) 和仅多糖条带 (DFP) 进行了三个判别分析测试，以探索 FTIR-ATR 在物种和生长位置之间分离的能力。DFL 模型能够很好地区分松树物种，而 DFP 模型能够区分物种和生长位置。DFT 模型基于涉及十二个 FTIR 波段的两个函数，实现了几乎完美的分离。物种之间的区分与 860 和 1655 cm-1 的条带有关，这在 P. sylvestris 样品中更强烈，而在 1425 和 1635 cm-1 处的条带，在 P. nigra 样品中更强烈。这些振动与木质素结构和多糖线性链的差异有关。生长位置之间的区别主要与多糖吸收有关：在 900、1085 和 1335 cm-1 处更能代表 Pn-LIN 样品，而在 1105 和 1315 cm-1 处主要与 Pn-LSA 样品相关。这些吸收与β-糖苷键（900 cm-1）、纤维素和半纤维素（C-O 键，1085 和 1105 cm-1）以及无定形/结晶纤维素的含量（1315 和 1335 cm-1）有关。