Wounds in patients with diabetes mellitus are one of the most prevalent impaired wounds in the world. Vascular endothelial growth factor (VEGF) is one of the most important proangiogenic mediators. Artificial dermal (AD) such as Pelnac^® has been shown, in humans and animal models, a great therapeutic potential in full-thickness skin wounds. We attempt to promote the wound healing in diabetic porcine models through combined use of AD and constant concentration of VEGF or VEGF concentration gradient. We created full-thickness excisional wounds in diabetic animal models. Analyzed the healing process through images, histology and immunohistochemistry. Results show that the combination of AD and concentration gradient of VEGF could provide an appropriate angiogenesis, improve granulation formation, increase epithelization and maintain the VEGF levels of the wound bed. Eventually accelerate the direct healing of diabetic wounds or make good preparation for secondary skin graft.

糖尿病患者的伤口是世界上最普遍的受损伤口之一。血管内皮生长因子（VEGF）是最重要的促血管生成介质之一。人工真皮（AD），如Pelnac ^®在人和动物模型中已显示出对全厚度皮肤伤口的巨大治疗潜力。我们试图通过结合使用AD和恒定浓度的VEGF或VEGF浓度梯度来促进糖尿病猪模型中的伤口愈合。我们在糖尿病动物模型中创建了全层切除伤口。通过图像，组织学和免疫组织化学分析愈合过程。结果表明，AD和VEGF浓度梯度的结合可提供适当的血管生成，改善肉芽形成，增加上皮形成并维持创面的VEGF水平。最终加速糖尿病伤口的直接愈合或为继发皮肤移植做好准备。