Bacteriophages of Acidovorax citrulli, the causal agent of bacterial fruit blotch, were isolated from 39 watermelon, pumpkin, and cucumber leaf samples collected from various regions of Korea and tested against 18 A. citrulli strains. Among the six phages isolated, ACP17 forms the largest plaque, and exhibits the morphology of phages in the Myoviridae family with a head diameter of 100 ± 5 nm and tail length of 150 ± 5 nm. ACP17 has eclipse and latent periods of 25 ± 5 min and 50 ± 5 min, respectively, and a burst size of 120. The genome of ACP17 is 156,281 base pairs with a G + C content of 58.7%, 263 open reading frames, and 4 transfer RNA genes. Blast search and phylogenetic analysis of the major capsid protein showed that ACP17 has limited homology to two Stentrophomonas phages, suggesting that ACP17 is a new type of Myoviridae isolated from A. citrulli.

中文翻译：

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细菌噬菌体病原体Acidovorax citrulli的噬菌体的首次分离和分子表征。

从韩国各个地区收集的39份西瓜，南瓜和黄瓜叶片样品中分离了Acidovorax citrulli细菌噬菌体（细菌水果斑点的病原体），并针对18株A. citrulli菌株进行了测试。在分离的六个噬菌体中，ACP17形成最大的噬菌斑，并在肌病毒科中表现出噬菌体的形态头直径为100±5 nm，尾长为150±5 nm的家族。ACP17的月食和潜伏期分别为25±5分钟和50±5分钟，爆发大小为120。ACP17的基因组为156,281个碱基对，G + C含量为58.7％，有263个开放阅读框，以及4个转移RNA基因。高炉搜索和主要衣壳蛋白的系统发育分析表明，ACP17具有同源性限于两个Stentrophomonas噬菌体，表明ACP17是一种新型的肌尾噬菌体科从隔离A. citrulli。