This study aimed to develop gel-creams from the lyophilised product of betamethasone dipropionate-loaded lipid-core nanocapsule suspensions and evaluated its efficacy in a model of contact dermatitis. The gel-creams were prepared and characterized followed by a study of in vitro drug penetration/permeation and its in vivo efficacy. The suspensions and lyophilised products showed nanometric size; the betamethasone content was 0.25 ± 0.01 mg/mL and the encapsulation efficiency was approximately 100%. The nanocapsules and redispersed powders presented control of the drug release. The gel-creams presented pH between 6.0-6.5 and exhibited non-Newtonian flow behavior, following the Herschel-Bulkley model. The skin penetration/permeation study indicated that betamethasone dipropionate can reach different skin layers. For in vivo efficacy, the contact dermatitis model was capable of causing tissue damage with changes in enzyme activities of the purinergic system in lymphocytes. The gel-creams showed the best dermatological and immunological efficacy and reduced oxidative damage in the evaluated tissues.

中文翻译：

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含有脱水二丙酸倍他米松纳米胶囊的凝胶霜的体内功效和评价。


本研究旨在利用二丙酸倍他米松脂质核心纳米胶囊悬浮液的冻干产品开发凝胶霜，并评估其在接触性皮炎模型中的功效。制备并表征凝胶霜，然后研究体外药物渗透/渗透及其体内功效。悬浮液和冻干产物呈现纳米尺寸；倍他米松含量为0.25±0.01 mg/mL，包封率约为100%。纳米胶囊和再分散粉末可以控制药物释放。凝胶霜的 pH 值在 6.0-6.5 之间，并表现出遵循 Herschel-Bulkley 模型的非牛顿流动行为。皮肤渗透/渗透研究表明二丙酸倍他米松可以到达不同的皮肤层。对于体内功效，接触性皮炎模型能够通过淋巴细胞中嘌呤能系统的酶活性的变化引起组织损伤。凝胶霜显示出最佳的皮肤病学和免疫学功效，并减少了所评估组织的氧化损伤。