BACKGROUND Acrolein (allyl Aldehyde) as one of smoke irritant exacerbates chronic airway diseases and increased in sputum of patients with asthma and chronic obstructive lung disease. But underlying mechanism remains unresolved. The aim of study was to identify protein expression in human lung microvascular endothelial cells (HMVEC-L) exposed to acrolein. METHODS A proteomic approach was used to determine the different expression of proteins at 8 h and 24 h after treatment of acrolein 30 nM and 300 nM to HMVEC-L. Treatment of HMVEC-L with acrolein 30 nM and 300 nM altered 21 protein spots on the two-dimensional gel, and these were then analyzed by MALDI-TOF MS. RESULTS These proteins included antioxidant, signal transduction, cytoskeleton, protein transduction, catalytic reduction. The proteins were classified into four groups according to the time course of their expression patterns such as continually increasing, transient increasing, transient decreasing, and continually decreasing. For validation immunohistochemical staining and Western blotting was performed on lung tissues from acrolein exposed mice. Moesin was expressed in endothelium, epithelium, and inflammatory cells and increased in lung tissues of acrolein exposed mice compared with sham treated mice. CONCLUSIONS These results indicate that some of proteins may be an important role for airway disease exacerbation caused by acrolein exposure.

中文翻译：

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暴露于丙烯醛后对 moesin 的蛋白质组学鉴定。

背景技术丙烯醛（烯丙基醛）作为烟雾刺激物之一，会加剧慢性气道疾病，并在哮喘和慢性阻塞性肺病患者的痰液中增加。但潜在的机制仍未解决。研究的目的是鉴定暴露于丙烯醛的人肺微血管内皮细胞 (HMVEC-L) 中的蛋白质表达。方法 蛋白质组学方法用于确定在 30 nM 和 300 nM 丙烯醛处理 HMVEC-L 后 8 小时和 24 小时蛋白质的不同表达。用 30 nM 和 300 nM 丙烯醛处理 HMVEC-L 会改变二维凝胶上的 21 个蛋白质点，然后通过 MALDI-TOF MS 分析这些点。结果这些蛋白质包括抗氧化剂、信号转导、细胞骨架、蛋白质转导、催化还原。蛋白质根据其表达模式的时间进程分为四组，如持续增加、瞬时增加、瞬时减少和持续减少。为了验证免疫组织化学染色和蛋白质印迹对来自丙烯醛暴露小鼠的肺组织进行。与假手术处理的小鼠相比，Moesin 在暴露于丙烯醛的小鼠的内皮、上皮和炎症细胞中表达，并在肺组织中增加。结论 这些结果表明，一些蛋白质可能在丙烯醛暴露引起的气道疾病恶化中起重要作用。为了验证免疫组织化学染色和蛋白质印迹对来自丙烯醛暴露小鼠的肺组织进行。与假手术处理的小鼠相比，Moesin 在暴露于丙烯醛的小鼠的内皮、上皮和炎症细胞中表达，并在肺组织中增加。结论 这些结果表明，一些蛋白质可能在丙烯醛暴露引起的气道疾病恶化中起重要作用。为了验证免疫组织化学染色和蛋白质印迹对来自丙烯醛暴露小鼠的肺组织进行。与假手术处理的小鼠相比，Moesin 在暴露于丙烯醛的小鼠的内皮、上皮和炎症细胞中表达，并在肺组织中增加。结论 这些结果表明，一些蛋白质可能在丙烯醛暴露引起的气道疾病恶化中起重要作用。