The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.

中文翻译：

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多功能二元载体中由双重35S和T7启动子驱动的苹果褪绿叶斑病病毒韩国新分离株感染性克隆的生成。

来自韩国的新的苹果绿化叶斑病病毒（ACLSV）分离株的全长序列存在差异，但与日本分离株A4的关系最为密切，核苷酸同一性为84％。将韩国CLSC分离株的全长cDNA克隆到噬菌体T7 RNA启动子和花椰菜花叶病毒35S启动子下游的二元载体中。使用T7启动子合成的体外转录本成功感染了藜藜（Chenopodium quinoa），在接种后20天（dpi）进行了检测，但没有产生明显的症状。通过农杆菌渗入接种了西方烟草和藜麦。在32 dpi时评估感染率。不奎奴亚藜C. quinoa植物通过农杆菌浸润感染，但感染了西方猪笼草。