Segregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of XcvΔscpBXv (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried out and the abundance of 187 proteins was altered. Among them, diverse transcriptional regulators involved in biofilm formation and virulence were abundant in the wild-type. We further showed that biofilm formation of XcvΔscpBXv was reduced. This study provides new insights into the functions of ScpBXv in bacterial replication and biofilm formation, which may contribute to the virulence of Xcv.

中文翻译：

---

功能和蛋白质组学分析揭示了ScpBXv参与了Xanthomonas campestris pv的细菌生长，毒力和生物膜形成。vesicatoria。

分离和凝结蛋白B（ScpB）对于在生物体中复制和分离至关重要。在此，我们报道ScpBXv（SCPB样蛋白质的功能黄单胞菌PV。vesicatoria使用表型和蛋白质组分析）。XcvΔscpBXv的生长（ScpBXv基因敲除突变体）在慢速和快速生长条件下在丰富的培养基中均降低，但与在植物模拟条件下的野生型相当。有趣的是，该突变体在番茄中的毒性远低于野生型，表明ScpBXv参与了毒性。为了研究ScpBXv相关的机制，进行了比较蛋白质组学分析，并改变了187种蛋白质的丰度。其中，在野生型中涉及生物膜形成和毒力的各种转录调节因子丰富。我们进一步发现，XcvΔ的生物膜形成scpBXv减少。这项研究提供了ScpBXv在细菌复制和生物膜形成中的功能的新见解，这可能有助于Xcv的毒性。