Prospecting of local grapevine (Vitis vinifera L.) germplasm revealed that Tunisia possesses a rich patrimony which presents diversified organoleptic characteristics. However, viral diseases seriously affect all local grapevine cultivars which risk a complete extinction. Sanitation programs need to be established to preserve and exploit, as a gene pool, the Tunisian vineyards areas. The presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA), were confirmed in a Tunisian grapevine cultivar using serological and molecular analyses. The association between GRSPaV and GVA viruses induces more rugose wood symptoms and damages. For this reason the cleansing of the infected cultivar is highly advisable. Direct and recurrent somatic embryos of cv. 'Hencha' were successfully induced from filament, when cultured on Chée and Pool (1987). based-medium, enriched with 2 mg 1^-1 of 2,4-dichlorophenoxyacetic acid and 2.5 mg 1^-1 of Thidiazuron, after 36 weeks of culture. After six months of acclimatization, RT-PCR carried on 50 somaplants confirmed the absence of GVA, GRSPa-V as well as GLRaV-3 viruses in all somaplants. Ampelographic analysis, based on eight OIV descriptors, was carried out on two years acclimated somaplants, compared to the mother plant. Results demonstrated that the shape and contours of 46 somaclones leaves are identical to mother plant leaves and four phenotypically off-type plants were observed. The healthy state of 100% 'Hencha' somaclones and the high percentage of phenotypically true-to-type plants demonstrate that somatic embryogenesis is a promising technique to adopt for grapevine viruses elimination.

中文翻译：

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通过体细胞胚发生和使用Ampelographic描述符对Somaclones进行表征，从突尼斯品种中消除与葡萄叶相关的病毒3，葡萄藤茎麻点相关病毒和葡萄病毒A。

对当地葡萄（Vitis vinifera L.）种质的勘探表明，突尼斯拥有丰富的遗产，具有多种感官特征。但是，病毒性疾病严重影响了所有可能完全灭绝的当地葡萄品种。需要建立卫生计划，以保护和利用突尼斯葡萄园地区作为基因库。所述的存在葡萄卷叶伴随病毒-3（葡萄卷叶病毒-3），葡萄茎痘病毒相关病毒（GRSPaV）和葡萄病毒A（GVA），已在突尼斯葡萄品种中使用血清学和分子分析得到证实。GRSPaV和GVA病毒之间的关联会引起更皱纹的木材症状和损害。因此，强烈建议清洗感染的品种。简历的直接和复发体细胞胚胎。当在Chéeand Pool（1987）上培养时，成功地从细丝中成功诱导出“ Hencha”。基质，富含2 mg 1 ^-1的2,4-二氯苯氧基乙酸和2.5 mg 1 ^-1培养36周后，加入Thi​​diazuron。适应六个月后，RT-PCR进行了50株植株，确认所有植株中均不存在GVA，GRSPa-V和GLRaV-3病毒。与母本植物相比，基于八种OIV描述符的Ampelography分析是在适应了两年的豆科植物上进行的。结果表明，46种豆科植物无性系叶片的形状和轮廓与母体植物叶片相同，并且观察到了4种表型异型植物。100％“ Hencha”生长激素的健康状态和表型上符合实际情况的植物的高比例表明，体细胞胚发生是一种用于消除葡萄病毒的有前途的技术。